Mouse embryonic fibroblasts (MEFs) from mice homozygous for this targeted mutation exhibit impaired migration, cell spreading, transferrin recycling and reduced cholesterol levels. These mice may be useful in studies of endocytic transport, intracellular trafficking and recycling of membrane proteins, and cholesterol homeostasis.
Mia Horowitz, Tel Aviv University
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ehd1 | EH-domain containing 1 |
The protein encoded by the Ehd1 (EH-domain containing 1) gene contains a protein-binding EH (EPS15 homology) domain and has a role in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Mice that are homozygous for this targeted mutation are viable and fertile. No gene product (protein) is detected by Western blot analysis of lung, heart, testis and skeletal muscle from homozygous mice. However, a truncated 64kDa protein is detected by Western blot analysis of brain tissue from homozygous mice. No gene product (mRNA) is detected in primary mouse embryonic fibroblasts (MEFs) from homozygotes by RT-PCR. Ehd1 deficient MEFs plated on fibronectin have impaired migration and cell spreading. MEFs from homozygotes also exhibit impaired transferrin recycling, lower overall levels of beta-1 integrins on the plasma membrane, abnormal focal adhesion formation, and lower cellular cholesterol and reduced lipid droplet size.
A targeting vector designed by Dr. Mia Horowitz (Tel Aviv University) containing a NEO cassette was inserted into exon 3, which resulted in disruption of part of exon 3, and all of exons 4 and 5. The construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were aggregated with morulae. The resulting chimeric males were bred to 129/SvEv or Swiss Webster females. The mice were then bred to ICR. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1, Mia Horowitz |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Ehd1- |
Gene Symbol and Name | Ehd1, EH-domain containing 1 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 19 |
Molecular Note | A neomycin resistance cassette was inserted into exon 3 such that it fused to the first 164 bp of that exon and replaced exons 4 and 5. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the STOCK Ehd1tm1Mhor/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023073 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Ehd1<tm1Mhor> |
Frozen Mouse Embryo | STOCK Ehd1<tm1Mhor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Ehd1<tm1Mhor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Ehd1<tm1Mhor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Ehd1<tm1Mhor>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.