Igkrs (immunoglobulin kappa chain recombining sequence; also called RS) encodes sequences that rearrange to inactivate Igk (the immunoglobulin kappa chain complex) in developing B cells. It carries a canonical Ig gene recombination signal, and rearranges by V(D)J recombination to V&kappa; elements and to sites in the J&kappa;-C&kappa; intron. RS recombination results in deletion of the C&kappa; exon and silencing of the Igk complex.
The recombination signal of Igkrs was removed in these targeted mutation mice, blocking RS-mediated Igk inactivation. Receptor editing and self-tolerance are impaired in homozygotes, in some cases leading to autoantibody formation. Animals make fewer B cells expressing lambda chain, whereas &lambda; versus &kappa; isotype exclusion is only modestly affected. The mice do not show autoimmunity, though they have slightly elevated IgM anti-DNA antibodies. Homozygotes are fully viable and fertile.

The recombination signal of Igkrs (immunoglobulin kappa chain recombining sequence; also called RS) was removed in these targeted mutation mice, blocking RS-mediated Igk inactivation. Receptor editing and self-tolerance are impaired in homozygotes, in some cases leading to autoantibody formation.

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