B6(C)-H2-K^bm8/LrpJ

Stock number: 023029
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These mice carry the spontaneous H2-K^bm8 mutation and may be useful in studies of MHC structure and function, and T cell development and function.

Detailed description

The H2-K^bm8 (H-2^bh) variant allele mutation arose spontaneously in a male C57BL/6Kh mouse and contains four amino acid substitutions. Homozygotes are viable and fertile. Mice congenic for H2 haplotypes are widely used in immunologic research and often vary in immune response and pathogen susceptibility from the recipient strain.

Development

The H2-K^bm8 mutation arose spontaneously in a male C57BL/6Kh mouse (Melvold et al. Immunogenetics. 1982;15(2):177-85). There are 4 amino acid substitutions in this mutation: at amino acid position 22 (Tyr to Phe); amino acid position 23 (Met to Ile); amino acid position 24 (Glu to Ser); and amino acid position 30 (Asp to Asn). The strain was maintained as homozygote on the C57BL/6 background and may contain incidental BALB/c. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: H2-K^bm8
Name: b haplotype mutation 8
MGI accession ID: MGI:3618255
Generation method: Spontaneous
Marker symbol: H2-K
Marker name: histocompatibility 2, K region
Chromosome: 17
Strain of origin: C57BL/6ByKh
Molecular note: Sequence analysis revealed that this mutation comprises 7 nucleotide substitutions within a 24 base pair stretch compared with the parental genome sequence. The consequence is 4 amino acid substitutions: at amino acid position 22 (Tyr to Phe); amino acid position 23 (Met to Ile); amino acid position 24 (Glu to Ser); and amino acid position 30 (Asp to Asn).