C57BL/6N-Tg(Camk2a-HBEGF)BNkza/J

Stock number: 023015
Research areas: Neurobiology Research, Research Tools

Description

fDTR-line-B mice express a conditional diphtheria toxin receptor (DTR) under the transcriptional control of the murine CamKIIα promoter. These mice may be useful for neuronal-specific cell ablation.

Detailed description

fDTR-line-B mice have a loxP-flanked alkaline phosphatase-polyadenylation signal upstream of a human diphtheria toxin receptor (DTR; heparin-binding epidermal growth factor-like growth factor (HBEGF)), both under the transcriptional control of the murine CamKIIα promoter. Transgene expression is seen at 8 weeks of age in the forebrain and partial midbrain. After 8 weeks of age in the dentate gyrus, mossy fibers, and hippocampal area CA1, with minimal expression in hippocampal area CA3. Homozygotes are viable, fertility has not been determined. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will express DTR in cre expressing tissues, making those cells susceptible to DT administration. fDTR-line-B mice have decreased DTR expression in CA3 pyramidal cells making them useful when avoiding CA3 pyramidal cell ablation.

Development

The fDTR transgene was designed with a loxP-flanked alkaline phosphatase-polyadenylation signal upstream of a human diphtheria toxin receptor (DTR; heparin-binding epidermal growth factor-like growth factor (HBEGF)) under the transcriptional control of the murine calcium/calmodulin-dependent protein kinase II alpha (CamKIIα) promoter. The transgene was microinjected into fertilized C57BL/6NTac oocytes, and mice from founder line B were bred to C57BL/6NTac mice. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) to establish the colony.

Allele

Symbol: Tg(Camk2a-HBEGF)BNKza
Name: transgene insertion B, Kazu Nakazawa
MGI accession ID: MGI:5523547
Generation method: Transgenic
Attributes: Conditional ready (e.g. floxed); Inserted expressed sequence
Synonyms: DTR^loxP; fDTR; fDTR-line-B
Marker symbol: Tg(Camk2a-HBEGF)BNKza
Marker name: transgene insertion B, Kazu Nakazawa
Chromosome: UN
Strain of origin: C57BL/6N
Expressed genes: HBEGF,heparin binding EGF like growth factor,human
Molecular note: A targeting construct contained the Camk2a promoter, a loxp flanked alkaline phosphates-pA stop cassette and the sequence for the human homolog of heparin-binding epidermal growth factor-like growth factor (HBEGF) which functions as a diphtheria toxin receptor (DTR) in mice. Lines A and B were generated with line B exhibiting reduced expression compared with line A. Upon cre-mediated excision of the floxed AP stop cassette, the DTR is expressed making it possible to ablate specific cell populations.