These transgenic mice express cre recombinase under the control of the mouse Rorc (RAR-related orphan receptor gamma; also called RORγt) promoter. Expression can be found in double positive thymocytes and their CD4+ and CD8+ single positive progeny as well as all αβ T cells of the spleen and lymphoid tissue inducer cells (LTi) and RORG+ innate lymphoid cells.
Dr. Dan R. Littman, New York University Medical Center
Genetic Background | Generation |
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N20+pN1F14
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
These transgenic mice express cre recombinase under the control of the mouse Rorc (RAR-related orphan receptor gamma; also called RORγt) promoter.
When crossed with Gt(ROSA)26Sor floxed stop GFP reporter mice, double positive thymocytes and their CD4+ and CD8+ single positive progeny express GFP, whereas double negative precursors do not. In the spleen, all αβ T cells express GFP in contrast to γδ T cells, B cells, NK cells, CD11c+ dendritic cells and CD11b+ myeloid cells, which do not express GFP.
The mouse Rorc gene encoded by Caltech's (CITB) BAC clone I15 was modified to express cre recombinase inserted immediately 3' of the translation start codon. The vector was injected into FVB embryos. Resultant mice were backcrossed to C57BL/6 for at least 20 generations by the donating laboratory.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre expression can be found in double positive thymocytes and their CD4+ and CD8+ single positive progeny as well as all αβT cells of the spleen and lymphoid tissue inducer cells and RORG+ innate lymphoid cells. |
Allele Name | transgene insertion 1, Dan R Littman |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Rorc(gammat)-CreTG; Rorc-cre; RORgammat-Cre |
Gene Symbol and Name | Tg(Rorc-cre)1Litt, transgene insertion 1, Dan R Littman |
Gene Synonym(s) | |
Promoter | Rorc, RAR-related orphan receptor gamma, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre expression can be found in double positive thymocytes and their CD4+ and CD8+ single positive progeny as well as all αβT cells of the spleen and lymphoid tissue inducer cells and RORG+ innate lymphoid cells. |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | The cre gene was inserted into exon 1 of Rorc contained in a mouse BAC. Cre expression is seen in double and single positive thymocytes but not double negative thymocytes. |
Hemizygotes are viable and fertile.
When using the B6.FVB-Tg(Rorc-cre)1Litt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #022791 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizyous or Non Carrier forTg(Rorc-cre)1Litt |
Frozen Mouse Embryo | B6.FVB-Tg(Rorc-cre)1Litt/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Rorc-cre)1Litt/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Rorc-cre)1Litt/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.FVB-Tg(Rorc-cre)1Litt/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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