B6.FVB-Tg(Rorc-cre)1Litt/J

Stock number: 022791
Research areas: Research Tools

Description

These transgenic mice express cre recombinase under the control of the mouse Rorc (RAR-related orphan receptor gamma; also called RORγt) promoter. Expression can be found in double positive thymocytes and their CD4⁺ and CD8⁺ single positive progeny as well as all αβ T cells of the spleen and lymphoid tissue inducer cells (LTi) and RORG⁺ innate lymphoid cells.

Detailed description

These transgenic mice express cre recombinase under the control of the mouse Rorc (RAR-related orphan receptor gamma; also called RORγt) promoter.

When crossed with Gt(ROSA)26Sor floxed stop GFP reporter mice, double positive thymocytes and their CD4⁺ and CD8⁺ single positive progeny express GFP, whereas double negative precursors do not. In the spleen, all αβ T cells express GFP in contrast to γδ T cells, B cells, NK cells, CD11c⁺ dendritic cells and CD11b⁺ myeloid cells, which do not express GFP.

Development

The mouse Rorc gene encoded by Caltech's (CITB) BAC clone I15 was modified to express cre recombinase inserted immediately 3' of the translation start codon. The vector was injected into FVB embryos. Resultant mice were backcrossed to C57BL/6 for at least 20 generations by the donating laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Tg(Rorc-cre)1Litt
Name: transgene insertion 1, Dan R Littman
MGI accession ID: MGI:3054098
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Rorc-cre)1Litt
Marker name: transgene insertion 1, Dan R Littman
Chromosome: UN
Strain of origin: FVB
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre expression can be found in double positive thymocytes and their CD4+ and CD8+ single positive progeny as well as all αβT cells of the spleen and lymphoid tissue inducer cells and RORG+ innate lymphoid cells.
Molecular note: The cre gene was inserted into exon 1 of Rorc contained in a mouse BAC. Cre expression is seen in double and single positive thymocytes but not double negative thymocytes.