These Palb2fl/fl mutant mice possess loxP sites flanking exons 2-3 of the partner and localizer of BRCA2 (Palb2) gene. PALB2 binds to and colocalizes with the breast cancer 2 early onset protein (BRCA2) in the nucleus where they act to repair double strand DNA breaks and suppress tumor formation. Palb2 deficiency has been associated with familial predisposition to breast and pancreatic cancers. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 2-3 deleted in the cre-expressing tissues.
For example, when bred to STOCK Tg(KRT14-cre)1Amc/J mice (Stock No. 004782) expressing Cre recombinase in the basal epithelium of the mammary ducts, skin, and oral mucosa, Palb2 deficiency results in mammary tumor formation with a long latency of 420 day. When these Palb2 deficient mice are bred to B6.129P2-Trp53tm1Brn/J mice (Stock No. 008462), coincident deletion of Trp53 accelerates mammary tumor formation (latency of 192 days).
A targeting vector was designed to insert a loxP site upstream of exon 2, and a second loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, downstream of exon 3 of the partner and localizer of BRCA2 (Palb2) gene. The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred with C57BL/6J females. Offspring were bred with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J transgenic mice (Stock No. 003946) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. Resulting Palb2fl/fl mice were bred to C57BL/6J mice for at least 2 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
|Allele Name||targeted mutation 1.1, David M Livingston|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Palb2, partner and localizer of BRCA2|
|Strain of Origin||(C57BL/6 x 129S4/SvJae)F1|
|Molecular Note||A targeting vector was designed to insert a loxP site upstream of exon 2, and a second loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, downstream of exon 3. Flp-mediated recombination removed the neomycin resistance cassette.|
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129-Palb2tm1.1Dli/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #022732 in your Materials and Methods section.