B6.129-Klra17^tm1.1Apma/J

Stock number: 022728
Product name: Ly49q1^lox knock-out
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These Ly49q1^lox/lox KO mice possess a loxP site in exon 2 of of the killer cell lectin-like receptor, subfamily A, member 17 (Klra17) gene, resulting in premature termination of transcription. This strain may be useful for studying MHC regulation of pDC cytokine responses to pathogens.

Detailed description

These Ly49q1^lox/lox KO mice possess a loxP site in exon 2 of the killer cell lectin-like receptor, subfamily A, member 17 (Klra17) gene, resulting in premature termination of transcription. LY49Q1 is a C-type lectin-like receptor expressed on plasmacytoid dendritic cells (pDCs). Ly49Q is specific for class I major histocompatibility complex (MHC) molecule, H-2K^b, and positively regulates IFN-α production by pDCs. Mice that are homozygous for this allele are viable and fertile. They exhibit a decrease in IFN-α and interleukin-12 (IL-12) production after challenge with agonists and pathogens. The percentage and total number of pDCs in mice older than 9 months is double that of wildtype littermates.

Development

A targeting vector was designed to insert a loxP-flanked neomycin resistance (neo) cassette, in reverse orientation, into exon 2 of the killer cell lectin-like receptor, subfamily A, member 17 (Klra17) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a CMV-Cre expression plasmid to delete the neo cassette. Resulting ES cells were injected into blastocysts and resulting chimeric males were bred with 129S1/SvImJ females. These Ly49q1^lox/lox mice were bred to C57BL/6J mice for at least 12 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Klra17^tm1.1Apma
Name: targeted mutation 1.1, Andrew P Makrigiannis
MGI accession ID: MGI:4357862
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Klra17
Marker name: killer cell lectin-like receptor, subfamily A, member 17
Chromosome: 6
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Transient cre expression in ES cells removed the neo cassette leaving a loxP site inserted into exon 2. The insertion interrupts the open reading frame and causes premature termination. The absence of protein expression was confirmed by flow cytometry on plasmacytoid and myeloid dendritic cells.