These Ly49q1lox/lox KO mice possess a loxP site in exon 2 of of the killer cell lectin-like receptor, subfamily A, member 17 (Klra17) gene, resulting in premature termination of transcription. This strain may be useful for studying MHC regulation of pDC cytokine responses to pathogens.
Andrew P Makrigiannis, Dalhousie University
These Ly49q1lox/lox KO mice possess a loxP site in exon 2 of the killer cell lectin-like receptor, subfamily A, member 17 (Klra17) gene, resulting in premature termination of transcription. LY49Q1 is a C-type lectin-like receptor expressed on plasmacytoid dendritic cells (pDCs). Ly49Q is specific for class I major histocompatibility complex (MHC) molecule, H-2Kb, and positively regulates IFN-α production by pDCs. Mice that are homozygous for this allele are viable and fertile. They exhibit a decrease in IFN-α and interleukin-12 (IL-12) production after challenge with agonists and pathogens. The percentage and total number of pDCs in mice older than 9 months is double that of wildtype littermates.
A targeting vector was designed to insert a loxP-flanked neomycin resistance (neo) cassette, in reverse orientation, into exon 2 of the killer cell lectin-like receptor, subfamily A, member 17 (Klra17) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a CMV-Cre expression plasmid to delete the neo cassette. Resulting ES cells were injected into blastocysts and resulting chimeric males were bred with 129S1/SvImJ females. These Ly49q1lox/lox mice were bred to C57BL/6J mice for at least 12 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1.1, Andrew P Makrigiannis|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Klra17, killer cell lectin-like receptor, subfamily A, member 17|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Transient cre expression in ES cells removed the neo cassette leaving a loxP site inserted into exon 2. The insertion interrupts the open reading frame and causes premature termination. The absence of protein expression was confirmed by flow cytometry on plasmacytoid and myeloid dendritic cells.|
When maintaining a live colony, homozygotes may be bred together.
When using the Ly49q1lox knock-out mouse strain in a publication, please cite the originating article(s) and include JAX stock #022728 in your Materials and Methods section.
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