These EedF/F mutant mice possess loxP sites flanking exons 3-6 of the embryonic ectoderm development (Eed) gene. This strain may be useful for studying chromatin condensation and gene silencing.
Stuart H Orkin, Harvard University
These EedF/F mice possess loxP sites flanking exons 3-6 of the embryonic ectoderm development (Eed) gene. EED is part of polycomb repressive complexes (PRC) 2. PRC2 is involved in transcriptional repression by the addition of three methyl groups to histone 3 during chromatin condensation. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-6 deleted in cre-expressing tissues.
For example, when crossed to mice expressing Cre recombinase in erythrocytes, Eed deficient offspring contain reduced numbers of erythrocytes reaching the R3 stage of differentiation.
A targeting vector was designed to insert a loxP site upstream of exon 3, and a single loxP site downstream of exon 6 of the embryonic ectoderm development (Eed) gene. A frt-flanked neomycin resistance (neo) cassette was inserted between exons 6 and 7. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were maintained on a mixed B6;129 background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Stuart Orkin|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Eed, embryonic ectoderm development|
|Strain of Origin||129S1/Sv|
|Molecular Note||A targeting vector was designed to insert a loxP site upstream of exon 3, and an frt-flanked neomycin resistance (neo) cassette was inserted between exons 6 and 7 followed immediately by a second loxP site.|
When maintaining a live colony mice homozygous for the floxed allele may be bred together.
When using the Eedflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #022727 in your Materials and Methods section.