These Wnt1-Cre2 transgenic mice express Cre recombinase under the control of the mouse Wnt1, wingless-related MMTV integration site 1, promoter and enhancer, and have applications in studies of cell lineage tracing in the developing neural crest and midbrain.
Dr. Philippe Soriano, Mount Sinai School of Medicine
Genetic Background | Generation |
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N7F3
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Allele Type |
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Transgenic (Recombinase-expressing, Null/Knockout) |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
These Wnt1-Cre2 transgenic mice express Cre recombinase under the control of the mouse Wnt1, wingless-related MMTV integration site 1, promoter and enhancer. The transgene integrated into chromosome 2 causing a 257 bp deletion and a 45 Kb inverted segment in exon 5 of the E2f1 (E2F transcription factor 1) gene. The inversion contains all of exon 5, but deletes 23 Kb including exons 6 and 7. The deletion results in a functional knock-out of E2f1 in homozygous mice. Founder line 20 has a copy number of greater than 1-3. Cre recombinase activity is detected in both the cardiac and cranial neural crest, specifically in the branchial arches and cardiac outflow tract. When Wnt1-Cre2 transgenic mice are bred to mice containing loxP site-flanked sequences, cre-mediated recombination results in deletion of the floxed sequences in the midbrain and developing neural tube of the resulting offspring. These Wnt1-cre2 transgenic mice do not exhibit the ectopic transgene expression
or midbrain enlargement reported for Tg(Wnt1-cre)11Rth mice (Stock Nos. 003829 / 009107) in Lewis et al. 2013 Dev Biol 379:229.
Of note: there are reports of Cre recombinase activity in both male and female germlines in the 129S4 congenic line. The frequency of this germline recombination also appears to be modified by genetic background. We will modify the strain description if necessary as published results become available.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic insert designed by Dr. Jeffrey Bush (while at Mount Sinai School of Medicine) containing Cre recombinase, under the control of the 1.3 kb 5' promoter and 5.5 kb 3' enhancer of the mouse Wnt1, wingless-related MMTV integration site 1 was injected into fertilized 129S4 mouse eggs. Founder line 2 was subsequently established. The transgene integrated into chromosome 2 causing a 257 bp deletion and a 45 Kb inverted segment in exon 5 of the E2f1 (E2F transcription factor 1) gene. The inversion contains all of exon 5, but deletes 23 Kb including exons 6 and 7. Founder line 20 has a copy number of greater than 1-3. The mice were then backcrossed to 129S4 for 4 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to 129S4/SvJaeJ (Stock No. 009104) at least once, and then backcrossed to C57BL/6J (Stock No. 000664) for 5 generations using a marker assisted protocol.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase activity is detected in both the cardiac and cranial neural crest, specifically in the branchial arches and cardiac outflow tract. |
Allele Name | transgene insertion 2, Philippe Soriano |
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Allele Type | Transgenic (Recombinase-expressing, Null/Knockout) |
Allele Synonym(s) | Tg(Wnt1-cre)2Sor; Wnt1-Cre; Wnt1-Cre2 |
Gene Symbol and Name | E2f1, E2F transcription factor 1 |
Gene Synonym(s) | |
Promoter | Wnt1, wingless-type MMTV integration site family, member 1, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase activity is detected in both the cardiac and cranial neural crest, specifically in the branchial arches and cardiac outflow tract. |
Strain of Origin | C57BL/6 x C3H |
Chromosome | 2 |
Molecular Note | A transgenic insert containing cre recombinase, under the control of the 1.3 kb 5' promoter and 5.5 kb 3' enhancer of the mouse Wnt1, wingless-related MMTV integration site 1 was injected into fertilized B6C3 hybrid mouse eggs. Founder line 2 inserted into the gene at 154561346-154561603 (Build GRCm38/mm10) resulting in a 257 bp deletion and a 45 Kb inverted segment in exon 5 of the gene. The inversion contains all of exon 5, but deletes 23 Kb including exons 6 and 7. Founder line 2 has a copy number of 1-3. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). At present, the Donating Investigator is attempting to make the strain homozygous on the 129S4 background, and testing fertility in homozygotes.
When using the B6 Wnt1-Cre2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #022501 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(Wnt1-cre)2Sor |
Frozen Mouse Embryo | B6.Cg-E2f1<Tg(Wnt1-cre)2Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-E2f1<Tg(Wnt1-cre)2Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-E2f1<Tg(Wnt1-cre)2Sor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-E2f1<Tg(Wnt1-cre)2Sor>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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