STOCK Cxcl12^tm2.1Sjm/J

Stock number: 022458
Product name: Cxcl12-dsRED KIKO
Research areas: Research Tools

Description

These Cxcl12-dsRED knock-in/knock-out targeted mutation mice express DsRedE2 from the endogenous Cxcl12 (chemokine (C-X-C motif) ligand 12), mouse promoter. DsRed (stained with anti-RFP) is primarily expressed by perivascular stromal cells and endothelial cells throughout the bone marrow. This strain is useful in marking cellular sources of CXCL12.

Detailed description

Cxcl2 (chemokine (C-X-C motif) ligand 12), expressed by perivascular stromal cells, endothelial cells, and osteoblasts, is a chemokine required for hematopoietic stem cell (HSC) maintenance and retention in the bone marrow. It also promotes the proliferation and maintenance of B-lineage progenitors, and common lymphoid progenitors.

These Cxcl12-dsRED knock-in/knock-out targeted mutation mice express DsRed from the endogenous Cxcl12 promoter. The DsRed knock-in produces a strong loss of function phenotype. Bright fluorescence can be detected either directly or through anti-RFP immunohistochemistry. DsRed is primarily expressed by perivascular stromal cells and endothelial cells throughout the bone marrow. This strain is useful in marking cellular sources of CXCL12.

Development

A targeting vector was designed to insert a DsRed Express 2 (Clontech)-polyA-Frt-Neo-Frt cassette in exon 2. The mutation was created in B6.Cg-Thy1^a-derived Bruce 4 embryonic stem (ES) cells. Chimaeric mice were bred with B6(Cg)-Tyr^c-2J/J (see Stock No. 000058) to obtain germline transmission. The Neo cassette was subsequently deleted by mating with Flpe mice (see Stock No. 003800). This strain was backcrossed to C57BL/Ka for at least 8 generations by the donating lab.

Allele

Symbol: Cxcl12^tm2.1Sjm
Name: targeted mutation 2.1, Sean J Morrison
MGI accession ID: MGI:5486673
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Cxcl12
Marker name: chemokine (C-X-C motif) ligand 12
Chromosome: 6
Strain of origin: B6.Cg-Thy1^a
Expressed genes: RFP,Red Fluorescent Protein,coral
Site of expression: The knocked-in reporter DsRed is expressed in perivascular stromal cells, endothelial cells, and osteoblasts.
Molecular note: A DsRedE2-polyA-Frt-Neo-Frt cassette was inserted into the second exon of Cxcl12.