NOD.129P2(B6)-Tcrd^tm1Mom/JsdJ

Stock number: 022395
Product name: NOD.Tcrd
Strain synonyms: TCRdelta-, C-delta-, delta-, delta⁰, gammadelta TCR-, TCRdelta KO
Research areas: Diabetes and Obesity Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Mice harboring this T-cell receptor delta chain (Tcrd) targeted mutation may be useful for studying immunology and gamma delta T-cell function. These mice are highly resistant to autoimmune diabetes.

Detailed description

Mice homozygous for the Tcrd^tm1Mom targeted mutation are viable and fertile. Gamma delta T-cell receptor expression is deficient in all adult lymphoid and epithelial organs. There is normal development of the alpha beta T-cell lineage. Patterns of CD4⁺CD8^- and CD4^-CD8⁺ alpha beta T-cells are apparently normal. Mice do not develop inflammatory bowel disease. On an NOD background, these mice are highly resistant to autoimmune diabetes in a gene dose-dependent manner.

Development

The Tcrd^tm1Mom targeted mutant strain was developed by Dr. Peter Mombaerts in the laboratory of Dr. Susumu Tonegawa at the Center for Cancer Research, Massachusetts Institute of Technology. A replacement targeting vector containing Pgk-neo was used which resulted in a 4 kb deletion encompassing most of the delta chain constant region coding sequences. These mice were backcrossed 12 times to C57BL/6J inbred mice (Stock No. 000664) and are available as Stock No. 002120. Subsequently, Dr. Adrian Hayday and Dr. Robert Tigelaar at Yale backcrossed these mice to NOD/ShiLtJ inbred mice (Stock No. 001976) for at least 11 generations. Dr. Jayne Danska at the Hospital for Sick Children received these mice and they have been maintained in her colony for at least 20 generations.

Allele

Symbol: Tcrd^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:1857257
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tcrd
Marker name: T cell receptor delta chain
Chromosome: 14
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin resistance gene cassette was used to disrupt the constant gene segment (designated C-delta). Flow cytometry showed that cells isolated from the lymphoid or epithelial organs of homozygous mice did not react with an anti-pan Tcrd mAb. Immunoprecipitation showed an absence of expression on the surface of thymocytes isolated from homozygous mutant embryos.