These mice possess loxP sites flanking exons 3 and 4 of the Ucp2, uncoupling protein 2 (mitochondrial, proton carrier), gene and have applications in studies related to mitochondrial oxidative stress.
Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)
Ucp2, uncoupling protein 2 (mitochondrial, proton carrier), encodes an inner mitochondrial membrane protein that reduces ATP production; has a role in thermogenesis, obesity and diabetes; and is a marker of mitohormesis, conferring protection to oxidative stress.
These mice possess loxP sites on either side of exons 3 and 4 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 and 4 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in MCH-expressing
neurons (see Stock No. 014099 for example), this mutant mouse strain may be useful in studies of regulation of glucose
A targeting vector designed by Dr. Bradford B. Lowell (Beth Israel Deaconess Medical Center and Harvard Medical School) containing an FRT-flanked kanamycin selection cassette and a loxP site was utilized in the construction of this mutant. This selection cassette was inserted into intron 4 of the targeted gene, and another loxP site was inserted into the second intron. This construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to transgenic mice (on the 129S4/SvJaeSor genetic background) expressing FLPe recombinase (Stock No. 003946) to remove the selection cassette. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony and remove the FLPe recombinase allele.
|Allele Name||targeted mutation 2.1, Bradford B Lowell|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Ucp2, uncoupling protein 2 (mitochondrial, proton carrier)|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A targeting construct was used to introduce loxP sites into intron 2 and intron 6 of Ucp2, as well as an FRT-flanked kanamycin selection cassette. Homologous recombination in W4 ES cells was used to generate the floxed allele. Chimeras were bred to Flp recombinase expressing mice to remove the selection marker cassette.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Ucp2lox mouse strain in a publication, please cite the originating article(s) and include JAX stock #022394 in your Materials and Methods section.