Female mice homozygous for the null Astl or ovastacin (astacin-like metalloendopeptidase (M12 family)) allele exhibit moderately reduced fecundity. This strain may be useful for studying post-fertilization sperm blocking, and sperm/oocyte interaction during fertilization and early development.
Jurrien Dean, NIH / NIDDK
Astl or ovastacin (astacin-like metalloendopeptidase (M12 family)) encodes an oocyte-specific metalloendoprotease involved in cleavage of the zona pellucida protein ZP2. Cleavage of ZP2 modifies the zona pellucida by destroying the sperm binding domain preventing post-fertilization binding and polyspermy. In the absence of ovastacin, sperm binds to two-cell Astl-null embryos. Homozygous mice are viable and fertile, although female mice exhibit moderately reduced fecundity. This strain may be useful for studying post-fertilization sperm blocking, sperm/oocyte interaction during fertilization and early development.
A targeting vector containing the neomycin resistance gene was used to replace exons 2-3, thus deleting the transcriptional and translation start sites. The construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to FVB female mice. Upon arrival, mice were bred to FVB/NJ for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Jurrien Dean|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Astl, astacin-like metalloendopeptidase (M12 family)|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Exons 2 and 3 of the gene, containing the transcriptional and translational start sites, were replaced by a neomycin resistance marker.|
Female mice exhibit moderately reduced fecundity. The donating investigator maintains this strain by heterozygote female x homozygote male or heterozygote x heterozygote matings.
When using the FVB;129S6-Astltm1Dean/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36900 in your Materials and Methods section.