B6.129S6(SJL)-Fgf21^tm1.2Djm/J

Stock number: 022361
Product name: Fgf21^loxP
Research areas: Diabetes and Obesity Research, Metabolism Research, Research Tools

Description

Fgf21^loxP mutant mice possess loxP sites flanking exons 1-3 of the fibroblast growth factor 21 (Fgf21) gene. These mice may be useful for studying the role of FGF21 in coordinating metabolic responses to fasting.

Detailed description

These Fgf21^loxP mice possess loxP sites flanking exons 1-3 of the fibroblast growth factor 21 (Fgf21) gene. FGF21 is an endocrine hormone, expressed in liver and pancreas, which stimulates glucose uptake in adipocytes. FGF21 induction in liver by peroxisome proliferator-activated receptor a (PPARa) during fasting induces fatty acid oxidation ketogenesis in liver. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 1-3 deleted in the cre-expressing tissues.

For example, when bred to B6.129S4-Meox2^tm1(cre)Sor/J mice (Stock No. 003755) expressing germ line Cre-recombinase, livers from resulting offspring have decreased beta-oxidation, ketogenesis, and gluconeogenesis under fasted conditions.



Of note, the knock-out version of this allele is available as Stock No. 033846.

Development

A targeting vector was designed to insert a loxP site upstream of exon 1, followed by a second loxP site and a frt-flanked neomycin resistance (neo) cassette downstream of exon 3 of the fibroblast growth factor 21 (Fgf21) gene. The construct was electroporated into 129S6/SvEvTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred to C57BL/6J females. Offspring were bred with B6;SJL-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 003800) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. These Fgf21^loxP mice were bred to C57BL/6 mice for at least 10 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Fgf21^tm1.2Djm
Name: targeted mutation 1.2, David J Mangelsdorf
MGI accession ID: MGI:5486224
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Fgf21^loxP
Marker symbol: Fgf21
Marker name: fibroblast growth factor 21
Marker synonyms: Fgf8c
Chromosome: 7
Strain of origin: 129S6/SvEvTac
Molecular note: A targeting vector was designed to insert a loxP site upstream of exon 1, followed by a second loxP site and a frt-flanked neomycin resistance (neo) cassette downstream of exon 3. Flp-mediated recombination removed the neo cassette.