B6.Cg-Tg(Arc-cre/ERT2)MRhn/CdnyJ

Stock number: 022357
Research areas: Neurobiology Research, Research Tools

Description

These mice express Cre-ER^T2 under the direction of the activity regulated cytoskeletal-associated protein (Arc) promoter/enhancer regions. They are useful in comparing activation of neuronal populations in the hippocampus during encoding and retrieval of memory.

Development

Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-429N15) containing the entire mouse activity regulated cytoskeletal-associated protein (Arc) gene. A CreER^T2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain), followed by a SV40 polyadenylation sequence, was inserted into the translation initiation site of Arc. This BAC was microinjected into fertilized C57BL/6J x CBA oocytes. Offspring from founder line M have been backcrossed to C57BL/6J mice for at least 15 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.

Allele

Symbol: Tg(Arc-cre/ERT2)MRhn
Name: transgene insertion M, Rene Hen
MGI accession ID: MGI:5607252
Generation method: Transgenic
Attributes: Recombinase-expressing; Inducible
Marker symbol: Tg(Arc-cre/ERT2)MRhn
Marker name: transgene insertion M, Rene Hen
Chromosome: UN
Strain of origin: C57BL/6J x CBA
Expressed genes: cre/ERT2,Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of expression: Hippocampus.
Molecular note: Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-429N15) containing the entire mouse activity regulated cytoskeletal-associated protein (Arc) gene. A CreERT2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand-binding domain), followed by an SV40 polyadenylation sequence, was inserted into the translation initiation site of the gene. Founder lines M and F were established and maintained.