These mice express Cre-ERT2 under the direction of the activity regulated cytoskeletal-associated protein (Arc) promoter/enhancer regions. They are useful in comparing activation of neuronal populations in the hippocampus during encoding and retrieval of memory.
Dr. Rene Hen, Columbia University
Christine Denny, Columbia University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-429N15) containing the entire mouse activity regulated cytoskeletal-associated protein (Arc) gene. A CreERT2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain), followed by a SV40 polyadenylation sequence, was inserted into the translation initiation site of Arc. This BAC was microinjected into fertilized C57BL/6J x CBA oocytes. Offspring from founder line M have been backcrossed to C57BL/6J mice for at least 15 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | Hippocampus. |
Allele Name | transgene insertion M, Rene Hen |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | ArcCreERT2 |
Gene Symbol and Name | Tg(Arc-cre/ERT2)MRhn, transgene insertion M, Rene Hen |
Gene Synonym(s) | |
Promoter | Arc, activity regulated cytoskeletal-associated protein, mouse, laboratory |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | Hippocampus. |
Strain of Origin | C57BL/6J x CBA |
Chromosome | UN |
Molecular Note | Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-429N15) containing the entire mouse activity regulated cytoskeletal-associated protein (Arc) gene. A CreERT2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain), followed by a SV40 polyadenylation sequence, was inserted into the translation initiation site of the gene. Founder lines M and F were established and maintained. |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the B6.Cg-Tg(Arc-cre/ERT2)MRhn/CdnyJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #022357 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Arc-cre/ERT2)MRhn |
Frozen Mouse Embryo | B6.Cg-Tg(Arc-cre/ERT2)MRhn/CdnyJ | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Arc-cre/ERT2)MRhn/CdnyJ | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Arc-cre/ERT2)MRhn/CdnyJ | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Arc-cre/ERT2)MRhn/CdnyJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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