This strain expresses a wildtype mouse Pknd (paroxysmal nonkinesiogenic dyskinesia) transgene. It presents no overt phenotype, but acts as a control for A7V/A9V mutant transgenic lines (see Stock No. 022146 and Stock No. 018410).
Louis Ptacek, University of California, San Francisco
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Bacterial artificial chromosome (BAC) RP24-112K19 containing the entire mouse paroxysmal nonkinesiogenic dyskinesia (Pnkd) gene, 62 Kb of flanking sequence 5' to the AATG start site, and an internal ribosomal entry site (IRES) followed by an enhanced red fluorescent protein (DsRED) inserted into the 3' UTR was microinjected into the pronucleus of C57BL/6XSJL F1 oocytes. Founder mice (line 445) were established and found to have an estimated 3 copies of the transgene. These WT-Tg transgenic mice were subsequently backcrossed to C57BL/6J for at least 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony. The DsRED insertion is believed to be non-functional.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression |
Allele Name | transgene insertion 445, Louis J Ptacek |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Tg(Pnkd)445Ljp; WT-Tg |
Gene Symbol and Name | Tg(Pnkd,-DsRed)445Ljp, transgene insertion 445, Louis J Ptacek |
Gene Synonym(s) | |
Promoter | Pnkd, paroxysmal nonkinesiogenic dyskinesia, mouse, laboratory |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Strain of Origin | (C57BL/6 x SJL)F1 |
Chromosome | UN |
Molecular Note | A BAC including 62 kb of sequence 5' to the unmutated Pnkd was used. An internal ribosome entry site (IRES) followed by an enhanced red fluorescent protein gene (IRES/DsRed) was introduced into the 3'UTR. Three founder lines, 445, 471, and 472, were produced. |
While maintaining a live colony, these mice are bred as hemizygotes.
When using the B6.Cg-Tg(Pnkd,-DsRed)445Ljp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #022147 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non carrier for Tg(Pnkd,-DsRed)445Ljp |
Frozen Mouse Embryo | B6.Cg-Tg(Pnkd -DsRed)445Ljp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Pnkd -DsRed)445Ljp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Pnkd -DsRed)445Ljp/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Pnkd -DsRed)445Ljp/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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