B6.Cg-Tg(Pnkd,-DsRed)445Ljp/J

Stock number: 022147
Research areas: Metabolism Research, Neurobiology Research

Description

This strain expresses a wildtype mouse Pknd (paroxysmal nonkinesiogenic dyskinesia) transgene. It presents no overt phenotype, but acts as a control for A7V/A9V mutant transgenic lines (see Stock No. 022146 and Stock No. 018410).

Development

Bacterial artificial chromosome (BAC) RP24-112K19 containing the entire mouse paroxysmal nonkinesiogenic dyskinesia (Pnkd) gene, 62 Kb of flanking sequence 5' to the AATG start site, and an internal ribosomal entry site (IRES) followed by an enhanced red fluorescent protein (DsRED) inserted into the 3' UTR was microinjected into the pronucleus of C57BL/6XSJL F1 oocytes. Founder mice (line 445) were established and found to have an estimated 3 copies of the transgene. These WT-Tg transgenic mice were subsequently backcrossed to C57BL/6J for at least 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony. The DsRED insertion is believed to be non-functional.

Allele

Symbol: Tg(Pnkd,-DsRed)445Ljp
Name: transgene insertion 445, Louis J Ptacek
MGI accession ID: MGI:5466586
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: Tg(Pnkd)445Ljp; WT-Tg
Marker symbol: Tg(Pnkd,-DsRed)445Ljp
Marker name: transgene insertion 445, Louis J Ptacek
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: RFP,Red Fluorescent Protein,coral
Molecular note: A BAC including 62 kb of sequence 5' to the unmutated Pnkd was used. An internal ribosome entry site (IRES) followed by an enhanced red fluorescent protein gene (IRES/DsRed) was introduced into the 3'UTR. Three founder lines, 445, 471, and 472, were produced.