These c-FLIPf mice possess loxP sites flanking exon 1 of the CASP8 and FADD-like apoptosis regulator (Cflar) gene and have applications in studies related to apoptosis regulation.
Ralph Budd, University of Vermont, College of Medicine
These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in thymocytes (see Stock No. 003802 for example), this mutant mouse strain may be useful in studies of T lymphocyte development and apoptosis.
A targeting vector designed by Drs. Nu Zhang and You-Wen He (Duke University) containing a loxP site and a FRT site flanked NEO cassette. This selection cassette was inserted upstream of exon 1 of the targeted gene, and another loxP site was inserted downstream of exon 1. This construct was electroporated into 129S6/SvEvTac derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting male chimeric animals were crossed to transgenic female mice (on the 129 genetic background) expressing FLPe recombinase (FLPeR mice). Mice that retained the loxP site flanked exon 1 were then backcrossed to C57BL/6 mice for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, You-Weh He|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||cflipf; c-FLIPf|
|Gene Symbol and Name||Cflar, CASP8 and FADD-like apoptosis regulator|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A targeting vector was designed to insert loxP sites flanking exon 1, which encodes amino acids 1-98. An FRT-flanked neo cassette was included in the vector and subsequently removed by crossing with mice expressing Flp reocmbinase.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129-Cflartm1Ywh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #022009 in your Materials and Methods section.