NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(CAG-EGFP)1Osb/SzJ

Stock number: 021937
Product name: NSG-EGFP
Strain synonyms: NSG CAG-EGFP
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

These NSG-EGFP mice combine the features of the NOD/ShiLtJ background, the severe combined immune deficiency mutation (scid) and IL2 receptor gamma chain deficiency. They have widespread EGFP expression from the transgene, allowing for visualization of allogeneic and xenogeneic grafts.

Detailed description

These compound mutant mice carry both the Prkdc^scid Il2rg^tm1Wjl mutations and a transgene harboring an enhanced green fluorescent protein (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer (CAG).

Mice hemizygous for the EGFP transgene exhibit a robust and widespread expression of green fluorescent protein. These mice can act as recipients of allogeneic and xenogeneic grafts, with the murine GFP showing distinction between murine cells and xenogeneic grafts. They can also act as donors of fluorescent-labeled cells, making them an ideal model for cell transfer experiments. Animals lacking the Prkdc gene and the X-linked Il2rg gene, and hemizygous for GFP are viable and fertile and exhibit the same immunodeficient characteristics of the parental NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ strain (Stock No. 005557). They lack mature T cells, B cells, or functional NK cells, and are deficient in cytokine signaling, leading to better engraftment of human hematopoietic stem cells and peripheral-blood mononuclear cells than any other published mouse strain. View our Resources for the NSG mouse model, including discussion forum, immunodeficient model comparison, and categorized, up-to-date references.

Some researchers report variable expression of GFP in lung tissue when measuring by immunohistochemical techniques and direct measurement of GFP fluorescence. Data generated at the Jackson Laboratory indicate that GFP is expressed in peripheral white blood cells in measurable amounts by flow cytometry.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

These NSG-EGFP mice were produced by breeding NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice (Stock No. 005557) mice with C57BL/6-Tg(CAG-EGFP)1Osb/J mice (Stock No. 003291). The resulting mice heterozygous for the Prkdc^scid allele Il2rg^tm1Wjl allele and hemizygous for the Tg(CAG-EGFP)1Osb transgene were crossed to NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice for at least 8 generations. Heterozygotes were interbred to produce mice homozygous for the Prkdc^scid allele, homozygous (females) or hemizygous (males) for the Il2rg^tm1Wjl allele, and hemizygous for the Tg(CAG-EGFP)1Osb transgene.

Allele

Symbol: Tg(CAG-EGFP)1Osb
Name: transgene insertion 1, Research Institute for Microbial Diseases, Osaka University
MGI accession ID: MGI:2686773
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(CAG-EGFP)1Osb
Marker name: transgene insertion 1, Research Institute for Microbial Diseases, Osaka University
Chromosome: 15
Strain of origin: C57BL/6
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: All tissues, with the exception of erythrocytes and hair, appear green under excitation light.
Molecular note: cDNA encoding enhanced green fluorescent protein (EGFP) was adjoined to the chicken beta actin promoter and cytomegalovirus enhancer. A bovine globin polyadenylation signal was also included in the construct.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Allele

Symbol: Il2rg^tm1Wjl
Name: targeted mutation 1, Warren J Leonard
MGI accession ID: MGI:1857455
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il2rg
Marker name: interleukin 2 receptor, gamma chain
Chromosome: X
Strain of origin: 129S4/SvJae
Site of expression: Primarily lymphoid cells.
Molecular note: A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.