These compound mutant mice carry both the Prkdcscid Il2rgtm1Wjl mutations and a transgene harboring an enhanced green fluorescent protein (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer (CAG).
Mice hemizygous for the EGFP transgene exhibit a robust and widespread expression of green fluorescent protein. These mice can act as recipients of allogeneic and xenogeneic grafts, with the murine GFP showing distinction between murine cells and xenogeneic grafts. They can also act as donors of fluorescent-labeled cells, making them an ideal model for cell transfer experiments. Animals lacking the Prkdc gene and the X-linked Il2rg gene, and hemizygous for GFP are viable and fertile and exhibit the same immunodeficient characteristics of the parental NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ strain (Stock No. 005557). They lack mature T cells, B cells, or functional NK cells, and are deficient in cytokine signaling, leading to better engraftment of human hematopoietic stem cells and peripheral-blood mononuclear cells than any other published mouse strain. View our
Resources for the NSG mouse model, including discussion forum, immunodeficient model comparison, and categorized, up-to-date references.
Some researchers report variable expression of GFP in lung tissue when measuring by immunohistochemical techniques and direct measurement of GFP fluorescence. Data generated at the Jackson Laboratory indicate that GFP is expressed in peripheral white blood cells in measurable amounts by flow cytometry.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
