These NSG-EGFP mice combine the features of the NOD/ShiLtJ background, the severe combined immune deficiency mutation (scid) and IL2 receptor gamma chain deficiency. They have widespread EGFP expression from the transgene, allowing for visualization of allogeneic and xenogeneic grafts.
Dr. Leonard D. Shultz, The Jackson Laboratory
Genetic Background | Generation |
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?+pN4
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Prkdc | protein kinase, DNA activated, catalytic polypeptide |
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Il2rg | interleukin 2 receptor, gamma chain |
Allele Type |
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Transgenic (Reporter) |
Starting at:
$121.87 Domestic price for male 4-week |
257.40 Domestic price for breeder pair |
These compound mutant mice carry both the Prkdcscid Il2rgtm1Wjl mutations and a transgene harboring an enhanced green fluorescent protein (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer (CAG).
Mice hemizygous for the EGFP transgene exhibit a robust and widespread expression of green fluorescent protein. These mice can act as recipients of allogeneic and xenogeneic grafts, with the murine GFP showing distinction between murine cells and xenogeneic grafts. They can also act as donors of fluorescent-labeled cells, making them an ideal model for cell transfer experiments. Animals lacking the Prkdc gene and the X-linked Il2rg gene, and hemizygous for GFP are viable and fertile and exhibit the same immunodeficient characteristics of the parental NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ strain (Stock No. 005557). They lack mature T cells, B cells, or functional NK cells, and are deficient in cytokine signaling, leading to better engraftment of human hematopoietic stem cells and peripheral-blood mononuclear cells than any other published mouse strain. View our
Resources for the NSG mouse model, including discussion forum, immunodeficient model comparison, and categorized, up-to-date references.
Some researchers report variable expression of GFP in lung tissue when measuring by immunohistochemical techniques and direct measurement of GFP fluorescence. Data generated at the Jackson Laboratory indicate that GFP is expressed in peripheral white blood cells in measurable amounts by flow cytometry.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
These NSG-EGFP mice were produced by breeding NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice (Stock No. 005557) mice with C57BL/6-Tg(CAG-EGFP)1Osb/J mice (Stock No. 003291). The resulting mice heterozygous for the Prkdcscid allele Il2rgtm1Wjl allele and hemizygous for the Tg(CAG-EGFP)1Osb transgene were crossed to NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice for at least 8 generations. Heterozygotes were interbred to produce mice homozygous for the Prkdcscid allele, homozygous (females) or hemizygous (males) for the Il2rgtm1Wjl allele, and hemizygous for the Tg(CAG-EGFP)1Osb transgene.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | All tissues, with the exception of erythrocytes and hair, appear green under excitation light. |
Allele Name | severe combined immunodeficiency |
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Allele Type | Spontaneous |
Allele Synonym(s) | SCID |
Gene Symbol and Name | Prkdc, protein kinase, DNA activated, catalytic polypeptide |
Gene Synonym(s) | |
Site of Expression | T and B lymphocytes. |
Strain of Origin | C.BKa-Ighb/Icr |
Chromosome | 16 |
Molecular Note | A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*). |
Allele Name | targeted mutation 1, Warren J Leonard |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | [KO]gammac; CD132-; gammac-; gc-; Il2rgtm1Wjll; IL2Rgammanull |
Gene Symbol and Name | Il2rg, interleukin 2 receptor, gamma chain |
Gene Synonym(s) | |
Site of Expression | Primarily lymphoid cells. |
Strain of Origin | 129S4/SvJae |
Chromosome | X |
Molecular Note | A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein. |
Mutations Made By | Dr. Warren Leonard, NHLBI, NIH |
Allele Name | transgene insertion 1, Research Institute for Microbial Diseases, Osaka University |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | (ACTbEGFP)10sb; beta-actin-GFP; GFP; pCAGGS-GFP; Ten(ACTbEGFP)1Osb; Tg(ACTbEGFP)1Osb; Tg(ACTB-EGFP)1Osb; Tg(CAG-EGFP)1Osb; TgN(ACTbEGFP)1Osb; TgN(beta-act-EFGP)01Osb |
Gene Symbol and Name | Tg(CAG-EGFP)1Osb, transgene insertion 1, Research Institute for Microbial Diseases, Osaka University |
Gene Synonym(s) | |
Promoter | CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | All tissues, with the exception of erythrocytes and hair, appear green under excitation light. |
Strain of Origin | C57BL/6 |
Chromosome | 15 |
Molecular Note | cDNA encoding enhanced green fluorescent protein (EGFP) was adjoined to the chicken beta actin promoter and cytomegalovirus enhancer. A bovine globin polyadenylation signal was also included in the construct. |
Mutations Made By | Dr. Masaru Okabe, Osaka University |
When maintaining a live colony, mice homozygous for both the Prkdcscid and Il2rgtm1Wjl alleles, and hemizygous for the Tg(CAG-EGFP)1Osb transgene may be bred to mice homozygous for the Prkdcscid and Il2rgtm1Wjl mutations and wildtype (noncarrier) for the Tg(CAG-EGFP)1Osb transgene. Similarly, mice homozygous for both the Prkdcscid and Il2rgtm1Wjl alleles and hemizygous for the Tg(CAG-EGFP)1Osb transgene may be bred to homozygous NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice (Stock No. 005557).
Animals homozygous for all three alleles are believed to be embryonic lethal. The Donating Investigator reports that mice homozygous for the Tg(CAG-EGFP)1Osb transgene are not viable.
See our NSG husbandry information for additional details. SPF (specific pathogen-free) conditions are recommended. Also see additional NSG housing information.
When using the NSG-EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #021937 in your Materials and Methods section.
Service/Product | Description | Price |
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Homozygous for Prkdc<scid> Homozygous for Hemizyogus for Il2rg<tm1Wjl>, Hemizygous or non carrier for Tg(CAG-EGFP)1Osb |
Frozen Mouse Embryo | NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl> Tg(CAG-EGFP)1Osb/SzJ | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl> Tg(CAG-EGFP)1Osb/SzJ | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl> Tg(CAG-EGFP)1Osb/SzJ | $3373.50 |
Frozen Mouse Embryo | NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl> Tg(CAG-EGFP)1Osb/SzJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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