Overview

Also Known As: NSG CAG-EGFP, NSG-EGFP

These NSG-EGFP mice combine the features of the NOD/ShiLtJ background, the severe combined immune deficiency mutation (scid) and IL2 receptor gamma chain deficiency. They have widespread EGFP expression from the transgene, allowing for visualization of allogeneic and xenogeneic grafts.

Donating Investigator

Dr. Leonard D. Shultz, The Jackson Laboratory

Genetic overview

Genetic Background	Generation
	?+pN4 (2019-06-12 00:00:00)

Tg(CAG-EGFP)1Osb

Allele Type
Transgenic (Reporter)

Prkdc^scid

Allele Type	Gene Symbol	Gene Name
Spontaneous	Prkdc	protein kinase, DNA activated, catalytic polypeptide

Il2rg^tm1Wjl

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Il2rg	interleukin 2 receptor, gamma chain

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools
Internal/Organ Research
Virology Research
Cancer Research

View All Research Applications

Base Price

Starting at:

$121.87 Domestic price for male

257.40 Domestic price for breeder pair

View Price List

Details

Detailed Description

These compound mutant mice carry both the Prkdc^scid Il2rg^tm1Wjl mutations and a transgene harboring an enhanced green fluorescent protein (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer (CAG).

Mice hemizygous for the EGFP transgene exhibit a robust and widespread expression of green fluorescent protein. These mice can act as recipients of allogeneic and xenogeneic grafts, with the murine GFP showing distinction between murine cells and xenogeneic grafts. They can also act as donors of fluorescent-labeled cells, making them an ideal model for cell transfer experiments. Animals lacking the Prkdc gene and the X-linked Il2rg gene, and hemizygous for GFP are viable and fertile and exhibit the same immunodeficient characteristics of the parental NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ strain (Stock No. 005557). They lack mature T cells, B cells, or functional NK cells, and are deficient in cytokine signaling, leading to better engraftment of human hematopoietic stem cells and peripheral-blood mononuclear cells than any other published mouse strain. View our
Resources for the NSG mouse model, including discussion forum, immunodeficient model comparison, and categorized, up-to-date references.

Some researchers report variable expression of GFP in lung tissue when measuring by immunohistochemical techniques and direct measurement of GFP fluorescence. Data generated at the Jackson Laboratory indicate that GFP is expressed in peripheral white blood cells in measurable amounts by flow cytometry.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

These NSG-EGFP mice were produced by breeding NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice (Stock No. 005557) mice with C57BL/6-Tg(CAG-EGFP)1Osb/J mice (Stock No. 003291). The resulting mice heterozygous for the Prkdc^scid allele Il2rg^tm1Wjl allele and hemizygous for the Tg(CAG-EGFP)1Osb transgene were crossed to NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice for at least 8 generations. Heterozygotes were interbred to produce mice homozygous for the Prkdc^scid allele, homozygous (females) or hemizygous (males) for the Il2rg^tm1Wjl allele, and hemizygous for the Tg(CAG-EGFP)1Osb transgene.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	All tissues, with the exception of erythrocytes and hair, appear green under excitation light.
Site of Expression	T and B lymphocytes.
Site of Expression	Primarily lymphoid cells.

Control Suggestions

Noncarrier

Approximate Controls

Additional Information

Considerations for Choosing Controls

Genetics

Tg(CAG-EGFP)1Osb

Allele Symbol: Tg(CAG-EGFP)1Osb

Allele Name	transgene insertion 1, Research Institute for Microbial Diseases, Osaka University
Allele Type	Transgenic (Reporter)
Allele Synonym(s)	transgene insertion 1, Research Institute for Microbial Diseases, Osaka University; Tg(CAG-EGFP)1Osb
Gene Symbol and Name	Tg(CAG-EGFP)1Osb, transgene insertion 1, Research Institute for Microbial Diseases, Osaka University
Gene Synonym(s)	Tg(ACTB-EGFP)1Osb; Tg(ACTbEGFP)1Osb; TgN(ACTbEGFP)1Osb; TgN(beta-act-EFGP)01Osb; (ACTbEGFP)10sb; GFP; Tg(ACTB-EGFP)1Osb
Promoter	CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter,
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	All tissues, with the exception of erythrocytes and hair, appear green under excitation light.
Strain of Origin	C57BL/6
Chromosome	15
Molecular Note	cDNA encoding enhanced green fluorescent protein (EGFP) was adjoined to the chicken beta actin promoter and cytomegalovirus enhancer. A bovine globin polyadenylation signal was also included in the construct.
Mutations Made By	Dr. Masaru Okabe, Osaka University

Prkdc^scid

Allele Symbol: Prkdc^scid

Allele Name	severe combined immunodeficiency
Allele Type	Spontaneous
Allele Synonym(s)	Prkdc^scid; severe combined immunodeficiency
Gene Symbol and Name	Prkdc, protein kinase, DNA activated, catalytic polypeptide
Gene Synonym(s)	AI326420; DNA-PK; DNA-PKcs; DNAPDcs; DNPK1; DOXNPH; doxorubicin nephropathy; DNAPK; expressed sequence AU019811; dxnph; dxnph; scid; IMD26; HYRC; HYRC1; p350; slip; XRCC7; severe combined immunodeficiency; expressed sequence AI326420; AU019811; DNA-PKC; DNAPKc
Site of Expression	T and B lymphocytes.
Strain of Origin	C.B-17
Chromosome	16
Molecular Note	A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon.

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Allele Name	targeted mutation 1, Warren J Leonard
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Il2rg^tm1Wjl; targeted mutation 1, Warren J Leonard
Gene Symbol and Name	Il2rg, interleukin 2 receptor, gamma chain
Gene Synonym(s)	common cytokine receptor gamma chain; CIDX; IMD4; gc; IL-2RG; P64; [g]c; SCIDX; SCIDX1; common gamma chain; gamma C receptor; gamma(c); CD132; Ab2-183; Cd132; gammaC
Site of Expression	Primarily lymphoid cells.
Strain of Origin	129S4/SvJae
Chromosome	X
Molecular Note	A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.
Mutations Made By	Dr. Warren Leonard, NHLBI, NIH

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Il2rg^tm1Wjl related

Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines
Cancer Research
- Growth Factors/Receptors/Cytokines

Genotype: Prkdc^scid related

Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
  - AIDS research tool

Research Tools
- Fluorescent Proteins
- Genetics Research
  - Tissue/Cell Markers
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B, T, and NK cell deficiency

Mammalian Phenotype Terms by Genotype

References

Additional References

Maykel J; Liu JH; Li H; Shultz LD; Greiner DL; Houghton J. 2014. NOD-scidIl2rg (tm1Wjl) and NOD-Rag1 (null) Il2rg (tm1Wjl) : a model for stromal cell-tumor cell interaction for human colon cancer. Dig Dis Sci 59(6):1169-79PubMed: 24798995 MGI: J:227975

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related

Additional - Tg(CAG-EGFP)1Osb related

Technical Support

Contact Technical Support

Genotyping Protocols
- End Point Analysis: Prkdc^scid End Point
- Probe: Il2rg^tm1Wjl-PROBE
- Probe: Fluorescent Proteins (Generic GFP)
- MELT: Tg(CAG-EGFP) Alternate2
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, mice homozygous for both the Prkdc^scid and Il2rg^tm1Wjl alleles, and hemizygous for the Tg(CAG-EGFP)1Osb transgene may be bred to mice homozygous for the Prkdc^scid and Il2rg^tm1Wjl mutations and wildtype (noncarrier) for the Tg(CAG-EGFP)1Osb transgene. Similarly, mice homozygous for both the Prkdc^scid and Il2rg^tm1Wjl alleles and hemizygous for the Tg(CAG-EGFP)1Osb transgene may be bred to homozygous NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice (Stock No. 005557).

Animals homozygous for all three alleles are believed to be embryonic lethal. The Donating Investigator reports that mice homozygous for the Tg(CAG-EGFP)1Osb transgene are not viable.

See our NSG husbandry information for additional details. SPF (specific pathogen-free) conditions are recommended. Also see additional NSG housing information.
- Additional Breeding and Husbandry Support
Mating System
- mice homozygous for both the Prkdc^scid and Il2rg^tm1Wjl alleles, and hemizygous for the Tg(CAG-EGFP)1Osb transgene may be bred to mice homozygous for the Prkdc^scid and Il2rg^tm1Wjl mutations and wildtype (noncarrier) for the Tg(CAG-EGFP)1Osb transgene
Citation
When using the NSG-EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #021937 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX12 (Maximum)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Not-For-Profit & Academic Pricing

Service	Genotype	Price
	Homozygous for Prkdc<scid> Homozygous for Hemizyogus for Il2rg<tm1Wjl>, Hemizygous or non carrier for Tg(CAG-EGFP)1Osb

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Strain(s) not available to companies or for-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: NSG CAG-EGFP, NSG-EGFP

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Tg(CAG-EGFP)1Osb

Allele Symbol: Tg(CAG-EGFP)1Osb

Prkdcscid

Allele Symbol: Prkdcscid

Il2rgtm1Wjl

Allele Symbol: Il2rgtm1Wjl

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Il2rgtm1Wjl related

Genotype: Prkdcscid related

Mammalian Phenotype Terms by Genotype

References

Additional References

Additional - Il2rgtm1Wjl related

Additional - Prkdcscid related

Additional - Tg(CAG-EGFP)1Osb related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Not-For-Profit & Academic Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Prkdc^scid

Allele Symbol: Prkdc^scid

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Genotype: Il2rg^tm1Wjl related

Genotype: Prkdc^scid related

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related