This hypomorphic gene trap demonstrates the role of Sec24d (Sec24 related gene family, member D (S. cerevisiae)) in embryonic development and protein secretion via COPII-coated vesicles.
David Ginsburg, University of Michigan
COPII-coated vesicles carry newly-synthesized proteins from the endoplasmic reticulem to the Golgi as part of the secretory pathway. SEC23/SEC24 protein heterodimers are an essential component of the COPII coat inner layer.
This hypomorphic gene trap derived from Bay Genomics embryonic stem (ES) cell clone RRR785 disrupts paralog Sec24d (Sec24 related gene family, member D (S. cerevisiae)). Homozygotes are embryonic lethal, but survive to mid-gestation (E13.5). Heterozygotes are viable and fertile.
Bay Genomics embryonic stem (ES) cell line RRR785, derived from 129P2/OlaHsd and expressing β-geo (a fusion of β-galactosidase and neomycin phosphotransferase II), was sequenced to locate the Sec24d gene trap insertion site at position 639 of intron 20. ES cells were cultured and microinjected into blastocysts. Resultant chimeras were backcrossed to C57BL/6J for at least 15 generations by the donating laboratory.
|Allele Name||gene trap RRR785, BayGenomics|
|Allele Type||Gene trapped (Reporter, Null/Knockout)|
|Gene Symbol and Name||Sec24d, Sec24 related gene family, member D (S. cerevisiae)|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||The beta-geo (lacZ and neomycin resistance cassette) containing gene trap vector inserted between exon 20 and 21. This allele is hypomorphic.|
Heterozygotes are viable and fertile, but homozygotes are not. Homozyogus embryos die sometime after embryonic day 13.5 (E13.5) and none survive to birth.
When using the B6.129P2-Sec24dGt(RRR785)Byg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021793 in your Materials and Methods section.