In this conditional mutant strain, exon 2 of the Rab11fip5 (RAB11 family interacting protein 5 (class I)) gene is flanked by loxP sites.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Rab11fip5 | RAB11 family interacting protein 5 (class I) |
Rab11Fip5 (RAB11 family interacting protein 5 (class I)) encodes a protein that interacts specifically with RAB11, which is key for endocytosis. In particular, Rab11Fip5 is required for transport in apical membranes. Preliminary investigations suggest a potential role for this gene in synaptic terminal exocytosis-endocytosis events and plasticity with potential relevance to autism.
In this conditional mutant strain, exon 2 of the Rab11fip5 gene is flanked by loxP sites. Mice homozygous for this allele are viable and fertile and produce normal levels of RAB11FIP5 protein. When crossed with a Cre-expressing strain or infected with Cre-expressing virus, the floxed allele is excised and expression of the mRNA is abolished.
A targeting vector placing a loxP-FRT-neomycin-FRT cassette in intron 1 and a loxP site in intron 2 was introduced to C57BL/6NTac-derived B6-3 embryonic stem (ES) cells. Resultant mice were maintained on C57BL/6 genetic background by the donating laboratory.
Allele Name | targeted mutation 1, Thomas C Sudhof |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | F5N-Rab11Fip5 conditional |
Gene Symbol and Name | Rab11fip5, RAB11 family interacting protein 5 (class I) |
Gene Synonym(s) | |
Strain of Origin | C57BL/6NTac |
Chromosome | 6 |
Molecular Note | A targeting vector placed a loxP-FRT-neomycin-FRT cassette in intron 1 and a loxP site in intron 2. |
Homozygous and heterozygous floxed mice are viable and fertile.
When using the C57BL/6-Rab11fip5tm1Sud/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021776 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Rab11fip5<tm1Sud> |
Frozen Mouse Embryo | C57BL/6-Rab11fip5<tm1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Rab11fip5<tm1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Rab11fip5<tm1Sud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Rab11fip5<tm1Sud>/J Frozen Embryo | $3373.50 |
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