C57BL/6-Rab11fip5^tm1Sud/J

Stock number: 021776
Research areas: Neurobiology Research, Research Tools

Description

In this conditional mutant strain, exon 2 of the Rab11fip5 (RAB11 family interacting protein 5 (class I)) gene is flanked by loxP sites.

Detailed description

Rab11Fip5 (RAB11 family interacting protein 5 (class I)) encodes a protein that interacts specifically with RAB11, which is key for endocytosis. In particular, Rab11Fip5 is required for transport in apical membranes. Preliminary investigations suggest a potential role for this gene in synaptic terminal exocytosis-endocytosis events and plasticity with potential relevance to autism.

In this conditional mutant strain, exon 2 of the Rab11fip5 gene is flanked by loxP sites. Mice homozygous for this allele are viable and fertile and produce normal levels of RAB11FIP5 protein. When crossed with a Cre-expressing strain or infected with Cre-expressing virus, the floxed allele is excised and expression of the mRNA is abolished.

Development

A targeting vector placing a loxP-FRT-neomycin-FRT cassette in intron 1 and a loxP site in intron 2 was introduced to C57BL/6NTac-derived B6-3 embryonic stem (ES) cells. Resultant mice were maintained on C57BL/6 genetic background by the donating laboratory.

Allele

Symbol: Rab11fip5^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:5468257
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Rab11fip5
Marker name: RAB11 family interacting protein 5 (class I)
Chromosome: 6
Strain of origin: C57BL/6NTac
Molecular note: A targeting vector placed a loxP-FRT-neomycin-FRT cassette in intron 1 and a loxP site in intron 2.