Exon 2 of the mouse Cxcl12 (chemokine (C-X-C motif) ligand 12) gene is flanked by loxP sites in this conditional targeted mutation strain. Cre recombinase-mediated excision of the floxed region enables tissue/cell-specific knockouts of the gene, useful in studies of hematopoietic stem cell maintenance or other CXCL12-dependent processes.
Daniel C. Link, Washington University Medical School
Genetic Background | Generation |
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N10pN3F1
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Cxcl12 | chemokine (C-X-C motif) ligand 12 |
Cxcl12 (chemokine (C-X-C motif) ligand 12) regulates many hematopoietic and non-hematopoietic cell types. CXCL12 is constitutively expressed at high levels in many bone marrow stromal cell types, where it contributes to both hematopoietic stem cell (HSC) and lymphoid progenitor maintenance. Exon 2 of the mouse gene is flanked by loxP sites in this conditional targeted mutation. Cre recombinase-mediated excision of the floxed region enables tissue/cell-specific knockouts of the gene, useful in studies of hematopoietic stem cell maintenance or other CXCL12-dependent processes.
Osx-Cre (Sp7-Cre, see Stock No. 006361)-mediated deletion of Cxcl12 from Sp7 transcription factor 7 (osterix)-expressing stromal cells, which include CXCL12-abundant reticular cells and osteoblasts, results in constitutive hematopoietic progenitor cell (HPC) mobilization and a loss of B-lymphoid progenitors, but HSC function is normal.
Tie2-Cre (Tek-Cre, see Stock No. 008863)- mediated deletion of Cxcl12 from endothelial cells results in a modest loss of long term repopulating activity.
Strikingly, deletion of Cxcl12 from mesenchymal progenitors using Prx1-Cre (Prrx1-Cre, see Stock No. 005584) is associated with a marked loss of HSCs, long term repopulating activity, HSC quiescence and common lymphoid progenitors.
Deletion of Cxcl12 from mineralizing osteoblasts via osteocalcin-Cre (BGLAP-Cre, see Stock No. 019509) crosses has no effect on HSCs or lymphoid progenitors.
A targeting vector incorporating loxP-exon 2-loxP-PGK-neomycin-loxP sequences was introduced to C57BL/6NTac-derived B6/BLU (lacZ reporter) embryonic stem (ES) cells. Mice carrying this targeted allele were crossed with B6.FVB congenic EIIa-Cre mice (see Stock No. 003724), and offspring carrying the Neo-excised allele were identified. This strain was backcrossed to C57BL/6 for 10 or more generations by the donating lab.
Allele Name | targeted mutation 1.1, Daniel C Link |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Cxcl12fl |
Gene Symbol and Name | Cxcl12, chemokine (C-X-C motif) ligand 12 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley |
Chromosome | 6 |
Molecular Note | A loxP site was inserted upstream of exon 2. A floxed neo cassette was inserted downstream of exon 2. Cre-mediated recombination removed the neo cassette and left exon 2 floxed. |
Homozygous and heterozygous mice are viable and fertile.
When using the B6(FVB)-Cxcl12tm1.1Link/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021773 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cxcl12<tm1.1Link> |
Frozen Mouse Embryo | B6(FVB)-Cxcl12<tm1.1Link>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(FVB)-Cxcl12<tm1.1Link>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(FVB)-Cxcl12<tm1.1Link>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(FVB)-Cxcl12<tm1.1Link>/J Frozen Embryo | $3373.50 |
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