These MRP8-Cre-ires/GFP transgenic mice may be useful in studies utilizing "Cre-lox" technology or fluorescent protein expression in granulocytes and granulocyte/macrophage progenitors (GMPs).
Emmanuelle Passegue, UC San Francisco
Genetic Background | Generation |
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N9+pN5F1
|
Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
Mice hemizygous for the MRP8-Cre-ires/GFP transgene are viable and fertile, with the human S100 calcium binding protein A8 (calgranulin A) (MRP8 or S100A8) promoter directing bicistronic Cre and EGFP protein expression to granulocytes and granulocyte/macrophage progenitors (GMPs). EGFP fluorescence and cre expression is evident in hemizygotes in 20% of granulocyte/macrophage progenitors (GMPs) and 100% of granulocytes during myeloid differentiation. When bred with mice containing a loxP-flanked sequence of interest, the resulting offspring can have Cre-mediated recombination of the flanked sequence in these cells.
In February 2021, The Jackson Laboratory examined a cohort of MRP8-Cre-ires/GFP hemizygous mice peripheral blood for flow cytometry analysis of GFP and the CD11b Ly6G macrophage marker. This confirmed GFP expression in MRP8-Cre-ires/GFP peripheral blood macrophages [view data].
The MRP8-Cre-ires/GFP transgene was designed to place a Cre recombinase gene, internal ribosomal entry site (IRES), and an enhanced green fluorescent protein (EGFP) gene all downstream of the human S100 calcium binding protein A8 (calgranulin A) (MRP8 or S100A8) promoter. This transgene was microinjected into the pronucleus of (C57BL/6 x C3H)F1 fertilized oocytes. Resulting transgenic mice were bred to C57BL/6J mice for at least 9 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | EGFP fluorescence and cre expression is evident in hemizygotes in 20% of granulocyte/macrophage progenitors and 100% of granulocytes during myeloid differentiation. |
Allele Name | transgene insertion 1, Irving L Weissman |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Mrp8creTg; MRP8-Cre-ires/GFP; Tg(S100A8-cre,-GFP)1Empa; Tg(S100A8-cre,-GFP)1Ilw |
Gene Symbol and Name | Tg(S100A8-cre,-EGFP)1Ilw, transgene insertion 1, Irving L Weissman |
Gene Synonym(s) | |
Promoter | S100A8, S100 calcium binding protein A8, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | EGFP fluorescence and cre expression is evident in hemizygotes in 20% of granulocyte/macrophage progenitors and 100% of granulocytes during myeloid differentiation. |
Strain of Origin | (C57BL/6 x C3H)F1 |
Chromosome | UN |
Molecular Note | The human promoter drives expression of cre followed by an IRES sequence and EGFP in granulocytes and a fraction of myelomonocytic progenitors. Two founders were generated (no line information given). |
When maintaining a live colony, hemizygous mice can be bred to wild-type (noncarrier) mice from the colony or C57BL/6J inbred mice (Stock No. 000664).
When using the MRP8-Cre-ires/GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #021614 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(S100A8-cre,-EGFP)1Ilw |
Frozen Mouse Embryo | B6.Cg-Tg(S100A8-cre -EGFP)1Ilw/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(S100A8-cre -EGFP)1Ilw/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(S100A8-cre -EGFP)1Ilw/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(S100A8-cre -EGFP)1Ilw/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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