These mice carry a knockout allele of the Trpc2 (transient receptor potential cation channel, subfamily C, member 2) gene. Homozygotes are deficient in vomeronasal organ (VNO) signaling and the detection of pheromonal cues, leading to social behavior modifications.
Catherine Dulac, Harvard University, HHMI
In mouse, the Trpc2 (transient receptor potential cation channel, subfamily C, member 2) gene is expressed primarily in the neurons of the vomeronasal organ (VNO). It is involved with processes of pheromone detection which impact social behaviors. The protein is not expressed by humans, which carry a pseudogene.
These targeted mutant mice carry a knockout of Trpc2. Western blot from the VNO shows elimination of expression. Homozygotes are deficient in VNO signaling and the detection of vomeronasal cues. Male mice deficient in TRPC2 expression fail to display male-male aggression, and they initiate sexual and courtship behaviors toward both males and females. Female mice deficient in TRPC2 show a reduction in maternal aggression and lactating behavior. They do display characteristics of male sexual and courtship behaviors such as mounting, pelvic thrust, solicitation, anogenital olfactory investigation, and emission of complex unltrasonic vocalizations towards male and female conspecific mice. In the adult, the number of V1R neurons in the homozygous mice is reduced by approximately 50% and the number of V2R-expressing neurons is reduced by approximately 75% in comparison with heterozygous mice.
Exons 7-10 were replaced by a floxed neomycin resistance cassette using 129S1/Sv-Oca2+Tyr+Kitl+-derived W9.5 embryonic stem (ES) cells. The strain was maintained on a mixed 129S1 and C57BL/6 genetic background by the donating laboratory.
|Allele Name||targeted mutation 1, Catherine Dulac|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Trpc2, transient receptor potential cation channel, subfamily C, member 2|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||The gene was disrupted by replacement of exons encoding transmembrane domains 4 and 5, part of a putative channel pore, and a conserved sequence motif (EWKFAR), with a floxed PGK-neo cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis of vomeronasal organ extracts.|
Heterozygotes and homozygotes are viable and fertile.
When using the B6;129S1-Trpc2tm1Dlc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021208 in your Materials and Methods section.