These TALH-HHO1 transgenic mice express human heme oxygenase (decycling) 1 (HMOX1) or HO-1 in the thick ascending loop of Henle as well as the renal medulla and cortex. In response to angiotensin II infusion, mice exhibit lower blood pressure than non-transgenic mice. This strain may be useful for studying the role of heme oxygenase in hypertension.
David Stec, University of Mississippi Medical Center
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
These transgenic mice express human heme oxygenase (decycling) 1 (HMOX1) or HO-1 cDNA under the control of the human uromodulin (UMOD) or TALH promoter. RT-PCR and microscopy confirm that HO-1 expression is elevated in the thick ascending loop of Henle (TALH) as well as the renal medulla and cortex, however heme oxygenase (HO) activity is increased only in the medulla. HO is a rate limiting enzyme involved in the catabolism of heme to biliverdin.
Hemizygous mice are viable, fertile, and normal in size. Transgenic mice exhibit lower blood pressure than non-transgenic mice in response to angiotensin II induced hypertension.
This strain may be useful for studying the role of heme oxygenase in hypertension.
The transgenic construct contains human heme oxygenase (decycling) 1 (HMOX1) or HO-1 cDNA under the direction of the human uromodulin (UMOD) or TALH promoter. This transgene was microinjected into fertilized C57BL/6 x SJL hybrid oocytes. Founders were established and backcrossed to C57BL/6J for more than 10 generations. Upon arrival, transgenic mice were bred to C57BL/6J for at least one generation to establish the colony
Expressed Gene | HMOX1, heme oxygenase 1, human |
---|---|
Site of Expression |
Allele Name | transgene insertion, David Stec |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | TALH-HHO1 |
Gene Symbol and Name | Tg(UMOD-HMOX1)#Stec, transgene insertion, David Stec |
Gene Synonym(s) | |
Promoter | UMOD, uromodulin, human |
Expressed Gene | HMOX1, heme oxygenase 1, human |
Strain of Origin | C57BL/6 x SJL |
Chromosome | UN |
Molecular Note | The human promoter drives reanl tubular-expression of the human cDNA. The pound symbol (#) is used whne no line is specified and/or lines are pooled. |
While maintaining a live colony, these mice are bred as hemizygotes. The donating investigator has not generated homozygous mice.
When using the B6.Cg-Tg(UMOD-HMOX1)#Stec/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36777 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.