These γ2I77lox mice contain the point mutation F77I and contain loxP sites flanking exon 4 of the gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 (Gabrg2) gene. GABAA receptors are rendered zolpidem-insensitive and allow cell type-specific ablations of the GABAA receptor γ2 subunit.
William Wisden, Imperial College London
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Gabrg2 | gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 |
A targeting vector was designed to insert a loxP site upstream of exon 4 followed by a frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of exon 4 of the gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 (Gabrg2) gene. A point mutation, resulting in a phenylalanine (F) to isoleucine (I) substitution at amino acid position 77, was introduced into exon 4. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 005703) to delete the neo cassette. Subsequently, these γ2I77lox mice were bred to C57BL/6J mice for two generations to remove the Flp-expressing transgene. These mice were maintained on a mixed background and also contained the Hdctm1(cre)Wwis allele, which was removed prior to shipping. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Allele Name | targeted mutation 1, Peter Wulff |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | gamma2I77lox |
Gene Symbol and Name | Gabrg2, gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 11 |
Molecular Note | Exon 4 was replaced with an exon containing a phenylalanine to isoleucine mutation at position 77 (F77I). This point mutation confers drug insensitivity to zolpidem. A loxP site was inserted in the upstream intron and a frt-flanked neomycin cassette followed by another loxP site was inserted in the downstream intron. Chimeric mice were mated with a Flp deleter strain to remove the neomycin cassette. Genomic sequencing of homozygous mice confirmed the presence of the F to I mutation. |
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Gabrg2tm1Wul/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021197 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gabrg2<tm1Wul> |
Frozen Mouse Embryo | STOCK Gabrg2<tm1Wul>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gabrg2<tm1Wul>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gabrg2<tm1Wul>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Gabrg2<tm1Wul>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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