These ARR2Pb-rtTA transgenic mice express reverse tetracycline-controlled transactivator directed by the rat Pbsn, probasin, promoter. These mice are a Tet-On tool that allows conditional, dox-inducible expression of genes in Pbsn expressing cells of the ventral prostate and may be useful for studying prostatic intraepithelial neoplasia (PIN) and prostatic cancer.
Xin Yuan, Beth Israel Deaconess Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Transactivator) |
These ARR2Pb-rtTA transgenic mice express reverse tetracycline-controlled transactivator, rtTA, reverse tetracycline regulated transactivator, followed by IRES sequence and EGFP sequence, under the control of the rat Pbsn, probasin, promoter. Transgene expression is detected in the ventral prostate. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice that are hemizygous for the transgene are viable and fertile. Homozygotes are viable. The Donating Investigator reports that the GFP expression is very low and cannot be detected (by direct fluorescence or by indirect immunostaining).
Dr. Xin Yuan, while at Beth Israel Deaconess Medical Center, Harvard Medical School, designed the Pb-rtTA-IRES-EGFP transgenic construct which contains rtTA, reverse tetracycline regulated transactivator, followed by IRES sequence and EGFP sequence, under the control of the rat Pbsn, probasin, promoter. This transgenic construct was injected into fertilized FVB/N mouse eggs. Founder line 42 was subsequently established. The mice were maintained on the FVB background. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony. A SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed 28 of 32 markers were fixed as FVB/NJ allele-type, and 4 of 32 markers were fixed as C57BL/6J allele-type. The mice were backcrossed to FVB/NJ (Stock No. 001800) for 6 generations (November 2015).
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
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Site of Expression | Transgene expression is detected in the ventral prostate. |
Allele Name | transgene insertion 42, Xin Yuan |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | ARR2Pb-rtTA |
Gene Symbol and Name | Tg(Pbsn-rtTA*M2)42Xy, transgene insertion 42, Xin Yuan |
Gene Synonym(s) | |
Promoter | Pbsn, probasin, rat |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | Transgene expression is detected in the ventral prostate. |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | The reverse tetracycline-controlled transactivator, rtTA, reverse tetracycline regulated transactivator, followed by IRES sequence and EGFP sequence, is under the control of the anterior prostate-specific rat promoter. Line 42 was generated. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to FVB/NJ inbred mice (Stock No. 001800). Homozygotes are viable.
When using the ARR2Pb-rtTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #021187 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(Pbsn-rtTA*M2)42Xy |
Frozen Mouse Embryo | FVB(B6)-Tg(Pbsn-rtTA*M2)42Xy/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB(B6)-Tg(Pbsn-rtTA*M2)42Xy/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB(B6)-Tg(Pbsn-rtTA*M2)42Xy/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB(B6)-Tg(Pbsn-rtTA*M2)42Xy/J Frozen Embryo | $3373.50 |
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