FVB(B6)-Tg(Pbsn-rtTA*M2)42Xy/J

Stock number: 021187
Product name: ARR2Pb-rtTA
Research areas: Research Tools

Description

These ARR2Pb-rtTA transgenic mice express reverse tetracycline-controlled transactivator directed by the rat Pbsn, probasin, promoter. These mice are a Tet-On tool that allows conditional, dox-inducible expression of genes in Pbsn expressing cells of the ventral prostate and may be useful for studying prostatic intraepithelial neoplasia (PIN) and prostatic cancer.

Detailed description

These ARR2Pb-rtTA transgenic mice express reverse tetracycline-controlled transactivator, rtTA, reverse tetracycline regulated transactivator, followed by IRES sequence and EGFP sequence, under the control of the rat Pbsn, probasin, promoter. Transgene expression is detected in the ventral prostate. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice that are hemizygous for the transgene are viable and fertile. Homozygotes are viable. The Donating Investigator reports that the GFP expression is very low and cannot be detected (by direct fluorescence or by indirect immunostaining).

Development

Dr. Xin Yuan, while at Beth Israel Deaconess Medical Center, Harvard Medical School, designed the Pb-rtTA-IRES-EGFP transgenic construct which contains rtTA, reverse tetracycline regulated transactivator, followed by IRES sequence and EGFP sequence, under the control of the rat Pbsn, probasin, promoter. This transgenic construct was injected into fertilized FVB/N mouse eggs. Founder line 42 was subsequently established. The mice were maintained on the FVB background. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony. A SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed 28 of 32 markers were fixed as FVB/NJ allele-type, and 4 of 32 markers were fixed as C57BL/6J allele-type. The mice were backcrossed to FVB/NJ (Stock No. 001800) for 6 generations (November 2015).

Allele

Symbol: Tg(Pbsn-rtTA*M2)42Xy
Name: transgene insertion 42, Xin Yuan
MGI accession ID: MGI:5461492
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(Pbsn-rtTA*M2)42Xy
Marker name: transgene insertion 42, Xin Yuan
Chromosome: UN
Strain of origin: FVB
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: Transgene expression is detected in the ventral prostate.
Molecular note: The reverse tetracycline-controlled transactivator, rtTA, reverse tetracycline regulated transactivator, followed by IRES sequence and EGFP sequence, is under the control of the anterior prostate-specific rat promoter. Line 42 was generated.