The entire open reading frame of the Mrgprb4 (MAS-related GPR, member B4) gene was replaced by an mtdTomato-2A-NLSCre-Frt-PGK-Neomycin-FRT cassette in these knockout/knockin allele mutant mice. When virally transduced, they are useful in studies of Mrgprb4-expressing sensory neurons. Constitutive expression of mtdTomato has been demonstrated in dorsal root ganglion (DRG) cells.
David J Anderson, California Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Mrgprb4 | MAS-related GPR, member B4 |
A rare population of unmyelinated sensory neurons express G protein-coupled receptor Mrgprb4 (MAS-related GPR, member B4). These neurons exclusively innervate hairy skin with large terminal arborizations that resemble the receptive fields of C-tactile afferents in humans.
The entire open reading frame of the gene was replaced by an mtdTomato-2A-NLSCre-Frt-PGK-Neomycin-FRT cassette in these knockout/knockin allele mutant mice. Mutant mice have no phenotype on their own, but when virally transduced (e.g. using Cre-dependent adeno-associated virus (AAV) to express genetically encoded calcium sensor GCaMP3.0 or hm3(Gq-coupled)DREADD) they are useful in studies of Mrgprb4-expressing sensory neurons. Constitutive Mrgprb4-specific expression of mtdTomato has been demonstrated in dorsal root ganglion (DRG) cells.
The entire open reading frame, encoded by a single exon, was replaced with an mtdTomato-2A-NLSCre-Frt-PGK-Neomycin-FRT cassette in 129S1/Sv-p+Tyr+Kitl +-derived CJ7 embryonic stem (ES) cells.
The membrane-tagged tdTomato (mtdTomato) contains the 8 amino acids of the MARCKS sequence (MGCCFSKT) fused to the N-terminus of the full-length tdTomato and was connected to a nuclear localization signal (NLS)-tagged Cre recombinase via an intervening foot-and-mouth disease virus 2A peptide (FMDV 2A or F2A) sequence.
This strain was backcrossed to C57BL/6N for five or more generations by the donating laboratory.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Constitutive Mrgprb4-specific expression of mtdTomato has been demonstrated in dorsal root ganglion cells. |
Allele Name | targeted mutation 3, David J Anderson |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Mrgprb4-cre; MrgprB4-mtdTomato-2A-NLScre-frt-PGK-neo-frt |
Gene Symbol and Name | Mrgprb4, MAS-related GPR, member B4 |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Constitutive Mrgprb4-specific expression of mtdTomato has been demonstrated in dorsal root ganglion cells. |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 7 |
Molecular Note | The entire open reading frame, encoded by a single exon, was replaced with an mtdTomato-2A-NLSCre-Frt-PGK-Neomycin-FRT cassette. The membrane-tagged tdTomato (mtdTomato) contains the 8 amino acids of the MARCKS sequence (MGCCFSKT) fused to the N-terminus of the full-length tdTomato and was connected to a nuclear localization signal (NLS)-tagged Cre recombinase via an intervening foot-and-mouth disease virus 2A peptide (FMDV 2A or F2A) sequence. |
Heterozygotes and homozygotes are viable and fertile.
When using the B6N.129S1-Mrgprb4tm3(cre)And/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021077 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Mrgprb4<tm3(cre)And> |
Frozen Mouse Embryo | B6N.129S1-Mrgprb4<tm3(cre)And>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S1-Mrgprb4<tm3(cre)And>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S1-Mrgprb4<tm3(cre)And>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.129S1-Mrgprb4<tm3(cre)And>/J Frozen Embryo | $3373.50 |
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