These Abca1fl/fl Abcg1fl/fl double floxed mice possess loxP sites flanking exons in the ATP-binding cassette, sub-family A (ABC1), member 1 (Abca1) gene and in the ATP-binding cassette, sub-family G (WHITE), member 1 (Abcg1) gene. This strain may be useful for studying cellular and plasma cholesterol homeostasis, and phospholipid transport.
Alan Tall, Columbia University
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Abca1 | ATP-binding cassette, sub-family A (ABC1), member 1 |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Abcg1 | ATP binding cassette subfamily G member 1 |
These Abca1fl/fl Abcg1fl/fl double floxed mice possess loxP sites flanking exons 45-46 of the ATP-binding cassette, sub-family A (ABC1), member 1 (Abca1) gene and loxP sites flanking exon 3 of the ATP-binding cassette, sub-family G (WHITE), member 1 (Abcg1) gene. Two loxP-flanked neomycin resistance cassettes are still present in these mice, and the donating investigator reports that they do not effect protein expression in macrophages. ABCA1 controls the assembly of phospholipids and free cholesterol with lipid-free Apolipoprotein A-I (apoA-I) to form high density lipoprotein (HDL) and also mediates cholesterol efflux to apolipoproteins and small, pre-beta HDL particles from peripheral cells. Mutations in Abca1 are associated with Tangier disease and familial hypoalphalipoproteinemia, which are characterized by near absence of plasma HDL and the accumulation of cholesterol esters in tissues. ABCG1 is involved in cholesterol and phospholipid efflux to large, mature HDL particles, and regulates cellular lipid homeostasis. Mice that are homozygous for these alleles are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have Abca1 exons 45-46 and Abcg1 exon 3 deleted in cre-expressing tissues.
For example, when crossed to B6.129P2-Lyz2tm1(cre)Ifo/J mice (Stock No. 004781) expressing Cre recombinase in the myeloid cell lineage, including monocytes, mature macrophages, and granulocytes, resulting mice display an increase in hematopoietic stem and multipotential progenitor cell (HSPC) mobilization and extramedullary hematopoiesis.
When crossed to C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J mice (Stock No. 007567) expressing Cre recombinase in dendritic cells, resulting mice display an increase in blood colony forming units, IL17 and IL23 secretion.
A targeting vector was designed to insert a loxP-flanked neomycin resistance (neo) cassette upstream of exon 45, and a single loxP site downstream of exon 46 of the ATP-binding cassette, sub-family A (ABC1), member 1 (Abca1) gene. The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred with C57BL/6J females. Resulting offspring were bred together to build a colony of Abca1fl/fl mice. These mice were backcrossed to C57BL/6J mice for at least 12 generations.
Another targeting vector was designed to insert a single frt site and a single loxP site upstream of exon 3, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3 of the ATP-binding cassette, sub-family G (WHITE), member 1 (Abcg1) gene. The construct was electroporated into B6(Cg)-Tyrc-2J/J-derived ES cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred with B6(Cg)-Tyrc-2J/J females. Resulting offspring were bred together to build a colony of Abcg1fl/fl mice. These mice were then bred to Abca1fl/fl mice, and double floxed mice were sent to The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. The Tyrc-2J gene was bred out of the colony.
Allele Name | targeted mutation 1, John S Parks |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Abca1fl; Abca1flox |
Gene Symbol and Name | Abca1, ATP-binding cassette, sub-family A (ABC1), member 1 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 4 |
Molecular Note | LoxP sites were inserted to flank exons 45 and 46, which encode the second nucleotide-binding fold. |
Allele Name | targeted mutation 1, Alan Tall |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Abcg1fl |
Gene Symbol and Name | Abcg1, ATP binding cassette subfamily G member 1 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 17 |
Molecular Note | A targeting vector was designed to insert a single frt site and a single loxP site upstream ofexon 3, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3. |
When maintaining a live colony, double homozygous mice may be bred together.
When using the Abca1fl Abcg1fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #021067 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Abca1<tm1Jp>, Heterozygous for Abcg1<tm1Tall> |
Frozen Mouse Embryo | B6.Cg-Abca1<tm1Jp> Abcg1<tm1Tall>/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Abca1<tm1Jp> Abcg1<tm1Tall>/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Abca1<tm1Jp> Abcg1<tm1Tall>/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Abca1<tm1Jp> Abcg1<tm1Tall>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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