Csf1r-iCre transgenic mice have codon optimized Cre recombinase (iCre) expression under direction of the murine colony stimulating factor 1 receptor promoter (Csf1r). iCre recombinase expression is evident in immune cells, including macrophages, dendritic cells and bone marrow derived granulocytes.
Jeffrey W Pollard, Albert Einstein College of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
Csf1r-iCre transgenic mice express a codon optimized Cre recombinase (iCre) under the control of the murine colony stimulating factor 1 receptor promoter (Csf1r). Csf1r encodes Cd115 which acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Homozygotes are viable and fertile. iCre expression is seen in in macrophages and dendritic cells of the liver, spleen, intestine, heart, kidney, and muscle. Expression is also seen in 80% of bone marrow derived macrophages, 50% of bone marrow derived granulocytes, and splenic T lymphocytes.
When crossed with a strain containing a loxP site-flanked sequence, iCre-mediated recombination results in deletion of the flanked sequence in these Csf1r+ cells.
For example, when bred to mice containing a conditional Stat3 allele (see B6.129S1-Stat3tm1Xyfu/J Stock No. 016923), the resulting mice develop inflammation in the intestines with tumor lesions similar to those observed in patients with inflammatory bowel disease.
A transgenic construct containing codon optimized cre recombinase (iCre) coding sequence under the control of the murine colony stimulating factor 1 receptor (Csf1r) promoter was introduced into FVB/N donor oocytes. Transgenic mice from founder line 1 were obtained and bred to FVB/NJ mice. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ (Stock No. 001800) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | iCre expression is seen in in macrophages and dendritic cells of the liver, spleen, intestine, heart, kidney, and muscle. Expression is also seen in 80% of bone marrow derived macrophages, 50% of bone marrow derived granulocytes, and splenic T lymphocytes. |
Allele Name | transgene insertion 1, Jeffrey W Pollard |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | cfms-icre; Tg(Csf1.icre)jwp; Tg(Csf1r-iCre)jwp |
Gene Symbol and Name | Tg(Csf1r-icre)1Jwp, transgene insertion 1, Jeffrey W Pollard |
Gene Synonym(s) | |
Promoter | Csf1r, colony stimulating factor 1 receptor, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | iCre expression is seen in in macrophages and dendritic cells of the liver, spleen, intestine, heart, kidney, and muscle. Expression is also seen in 80% of bone marrow derived macrophages, 50% of bone marrow derived granulocytes, and splenic T lymphocytes. |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | A transgenic construct was made with the icre (improved cre) sequence inserted downstream of the colony-stimulating factor-1 receptor (Csf1r) promoter. The construct was injected into FVB embryos. One of three founders transmitted the transgene to offspring and a line was established from this founder. |
When maintaining a live colony, homozygous mice may be bred together.
When using the FVB-Tg(Csf1r-icre)1Jwp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021024 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(Csf1r-icre)1Jwp |
Frozen Mouse Embryo | FVB-Tg(Csf1r-icre)1Jwp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Csf1r-icre)1Jwp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Csf1r-icre)1Jwp/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(Csf1r-icre)1Jwp/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.