STOCK Tg(Cp-HIST1H2BB/Venus)47Hadj/J

Stock number: 020942
Product name: CBF:H2B-Venus
Strain synonyms: STOCK Tg(Cp-HIST1H2BB/Venus)#Hadj/J
Research areas: Research Tools

Description

This CBF:H2B-Venus transgenic strain serves as a single cell resolution Notch signaling reporter and has applications in non-invasive and quantitative live imaging of canonical (CBF1-mediated) Notch signaling activity.

Detailed description

As a result of activation of the NOTCH signaling pathway, the transcriptional regulator, CBF1 (C promoter binding factor 1), binds with the cleaved intracellular domain of Notch(NICD). The NICD-CBF1 complex then acts with co-activator mastermind-like (MAML) to promote transcription of various target genes. These transgenic mice express a nuclear-localized fluorescent protein, the YFP variant Venus, under the control of multiple CBF1 (C promoter binding factor 1) binding sites and the basal simian virus 40 (SV40) promoter (also called CBFRE). YFP Venus expression is consistent with canonical (CBF1-mediated) Notch activity, acting as a single cell resolution Notch signaling reporter. In early postimplantation embryonic development transgene expression is detected in the epiblast of the peri-implantation embryos (approximately E5.0-5.25), in the anterior epiblast of E6.5 embryos, in the headfolds and in the blood islands (maturing erythrocytes surrounded by endothelial cells) of approximately E8.0 embryos. At mid-gestation, transgene expression is detected mostly in the vasculature: dorsal aortic endothelial cells, umbilical vessels, pharyngeal arch arteries, for example. At age E14.5, transgene expression is detected in the retinal pigmented epithelium, cuboidal epithelium of the anteriour lens, lung mesenchymal cells, developing heart valve cells, and other vascular cells. Postnatally, reporter expression is detected predominantly in the vasculature, as well as the lung interstitial mesenchymal cells, kidney, adrenal gland and cardiac and skeletal muscle cells. The transgene is expressed in the cerebellum, brain stem, telencephalon, mesencephalon, olfactory bulbs, and ganglia. On this mixed STOCK background, homozygotes are viable and fertile.

Development

The CBF:H2B-Venus transgenic insert designed by Dr. Anna-Katerina Hadjantonakis (Sloan-Kettering Institute) containing the HIST1H2BB/Venus, histone cluster 1, H2bb, human and Yellow Fluorescent Protein, Venus variant, fusion gene, under the control of 4 tandem copies of the Cp, CBF1 (C promoter binding factor 1) binding site consensus sequence upstream of the basal simian virus 40 (SV40) promoter, was injected into FVB pronuclei. Founder line 47 (also known as founder line 5) was subsequently established. The mice were crossed to CD1 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.

Allele

Symbol: Tg(Cp-HIST1H2BB/Venus)47Hadj
Name: transgene insertion 47, Anna-Katerina Hadjantonakis
MGI accession ID: MGI:5487759
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Cp-HIST1H2BB/Venus)47Hadj
Marker name: transgene insertion 47, Anna-Katerina Hadjantonakis
Chromosome: UN
Strain of origin: FVB
Expressed genes: YFP,Yellow Fluorescent Protein,jellyfish
Site of expression: In early postimplantation embryonic development transgene expression is detected in the epiblast of the peri-implantation embryos (approximately E5.0-5.25), in the anterior epiblast of E6.5 embryos, in the headfolds and in the blood islands (maturing erythrocytes surrounded by endothelial cells) of approximately E8.0 embryos. At mid-gestation, transgene expression is detected mostly in the vasculature: dorsal aortic endothelial cells, umbilical vessels, pharyngeal arch arteries, for example. At age E14.5, transgene expression is detected in the retinal pigmented epithelium, cuboidal epithelium of the anteriour lens, lung mesenchymal cells, developing heart valve cells, and other vascular cells. Postnatally, reporter expression is detected predominantly in the vasculature, as well as the lung interstitial mesenchymal cells, kidney, adrenal gland and cardiac and skeletal muscle cells. The transgene is expressed in the cerebellum, brain stem, telencephalon, mesencephalon, olfactory bulbs, and ganglia.
Molecular note: The transgene contains the HIST1H2BB/Venus, histone cluster 1, H2bb, human and Yellow Fluorescent Protein, Venus variant, fusion gene, under the control of 4 tandem copies of the Cp, CBF1 (C promoter binding factor 1) binding site consensus sequence of the Epstein-Barr virus major latency C promoter (Cp) upstream of the basal simian virus 40 (SV40) promoter. Lines 5 and 47 was generated. The transgene is expressed in the cerebellum, brain stem, telencephalon, mesencephalon, olfactory bulbs, and ganglia.