B6.129(C)-Igfals^tm1Yrb/J

Stock number: 019926
Research areas: Developmental Biology Research, Internal/Organ Research

Description

In this strain a NEO cassette replaces exon 1 and a portion of exon 2, encoding the first 435 amino acids and 16 or 19 leucine repeats of the insulin-like growth factor binding protein, acid labile subunit Igfals gene. These ALS KO mice exhibit skeletal defects and may be useful in studies of Acid-Labile Subunit Deficiency.

Development

A targeting vector containing a NEO cassette was used to disrupt exon 1 and 1300 bp of exon 2, which encodes the first 435 amino acids and 16 leucine repeats. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric animals were crossed to BALB/c mice, and then backcrossed to C57BL/6J for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Igfals^tm1Yrb
Name: targeted mutation 1, Yves R Boisclair
MGI accession ID: MGI:2386180
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: ALSKO
Marker symbol: Igfals
Marker name: insulin-like growth factor binding protein, acid labile subunit
Marker synonyms: ACLSD; Albs; Als
Chromosome: 17
Strain of origin: 129S4/SvJae
Molecular note: The sequences encoding the first 435 amino acids (including 16 out of 19 leucine repeats) corresponding to exon 1, intron 1, and part of exon 2 were replaced by a neomycin resistance cassette. Northern blot analysis showed an absence of gene transcripts in liver and kidneys of homozygous mutant animals. Plasma proteins from homozygous animals were unable to form cross-linking complexes which is consistent with deletion of the 16 leucine repeats.