These transgenic mice express the human MCL1 under the direction of the human endogenous promoter. Mice exhibit enlarged spleens, increased total splenocyte number, lymph node enlargement and a high probability of developing lymphomas. This transgenic mouse strain may be useful in studies related to tumorigenesis and inhibition of tumorigenesis in the presence of a viability-promoting BCL2 family member.
Ruth W. Craig, Dartmouth Medical School
A transgenic construct containing the entire human MCL1 gene, including the endogenous promoter, was microinjected into B6;SJLF1 fertilized oocytes. The donating investigator reports that founder line 8 was established and backcrossed to C57BL/6NTac for more than 10 generations (see SNP note below). Upon arrival, transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
|Expressed Gene||MCL1, myeloid cell leukemia 1, human|
|Site of Expression|
|Allele Name||transgene insertion 8, Ruth W Craig|
|Allele Type||Transgenic (Humanized sequence, Inserted expressed sequence)|
|Gene Symbol and Name||Tg(MCL1)8Caig, transgene insertion 8, Ruth W Craig|
|Promoter||MCL1, myeloid cell leukemia 1, human|
|Expressed Gene||MCL1, myeloid cell leukemia 1, human|
|Strain of Origin||(C57BL/6 x SJL)F1|
|Molecular Note||The transgene contains the entire human MCL1 gene, including the endogenous MCL1 promoter. Hemizygous transgenic mice expressed human MCL1 in bone marrow, lymph node, thymus, and spleen (both B- and T-cell populations). Low levels of transgene expression were found in the kidney, small intestine, uterus, lung and liver.|
|Mutations Made By|| |
Ruth Craig, Dartmouth Medical School
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