STOCK Dpf3^tm1.1Grc/J

Stock number: 019481
Product name: BAF45C KO
Research areas: Cardiovascular Research, Neurobiology Research

Description

These mice carry a germline knockout allele of the Dpf3 (BAF45c) gene which is normally expressed in the brain, heart, skeletal muscle and the kidney.

Detailed description

This strain carries a germline knockout allele of the Dpf3 (D4, zinc and double PHD fingers, family 3; also known as BAF45c) gene created through a deletion of exon 4. Mutant animals have no phenotype, but may when placed in combination with other BAF gene mutations.

Development

A loxP-FRT-neomcyin-FRT cassette was introduced to intron 3 and a single loxP site was placed in intron 4 using an unspecified embryonic stem cell line. Exon 4 and the neomycin cassette were excised through crosses with a germline Cre strain of unknown genetic background. The donating laboratory believes the strain is a mix of C57BL/6 and 129.

Allele

Symbol: Dpf3^tm1.1Grc
Name: targeted mutation 1.1, Gerald R Crabtree
MGI accession ID: MGI:5440860
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Dpf3
Marker name: D4, zinc and double PHD fingers, family 3
Chromosome: 12
Strain of origin: Not Specified
Molecular note: A loxP-FRT-neomcyin-FRT cassette was introduced to intron 3 and a single loxP site was placed in intron 4 using an unspecified embryonic stem cell line. Cre-mediated recombination removed exon 4 and the neomycin cassette.