B6(Cg)-Tg(Lck-Cd1d1)1Aben/J

Stock number: 019418
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These transgenic mice express full-length mouse Cd1d1 (CD1d1 antigen) cDNA under the regulatory control of the Lck (lymphocyte protein tyrosine kinase) promoter. The transgene is faithfully expressed in the T cell lineage.

Detailed description

These transgenic mice express full-length mouse Cd1d1 (CD1d1 antigen) cDNA under the regulatory control of the Lck (lymphocyte protein tyrosine kinase) promoter. The transgene is faithfully expressed in the T cell lineage (reported fold increase in thymus expression: CD4⁺(6x), CD8⁺ (4x), CD4⁺CD8⁺ (33x), CD4^-CD8^- (36x); in spleen CD4⁺ (4x), CD8⁺ (7.5x)).

Crosses with Cd1d1/Cd1d2 knockout mice to generate compound mutant lines were done to demonstrate that cortical thymocytes provide cell type-specific signals that lead to NKT cell differentiation.

Development

A transgenic vector was designed to carry full-length mouse Cd1d1 cDNA inserted into a rabbit β-globin cassette under the regulatory control of the proximal Lck promoter. The transgenic vector was introduced to C57BL/6 embryos. Resultant mice were crossed with C57BL/6 background Cd1d1 knockout mice. The Cd1d1 knockout allele was later bred away leaving only the transgene. This strain was maintained on a C57BL/6 genetic background by the donating laboratory. This line corresponds to the Lck/low founder reported in Wei et al., J. EXP. MED. 2005, 202: 239-48.

Allele

Symbol: Tg(Lck-Cd1d1)1Aben
Name: transgene insertion 1, Albert Bendelac
MGI accession ID: MGI:3589763
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Lck-Cd1d1)1Aben
Marker name: transgene insertion 1, Albert Bendelac
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: Cd1d1,CD1d1 antigen,mouse, laboratory
Molecular note: The Cd1d1 coding sequence was inserted into the second exon of the betaglobin expression cassette and subsequently placed under the control of the proximal Lck promoter. Expression of the Cd1d1 cDNA was demonstrated in the T cell lineage.
General note: The construct was microinjected into fertilized NOD oocytes as well.