Disruption of Spo11 (SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)) leads to severe gonadal abnormalities in both males and females due to defective meiosis.
Maria Jasin, Memorial Sloan-Kettering Cancer Center
Scott Keeney, Memorial Sloan-Kettering Cancer Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Spo11 | SPO11 meiotic protein covalently bound to DSB |
Disruption of Spo11 (SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)) leads to severe gonadal abnormalities in both males and females due to defective meiosis.
These mice carry a targeted deletion of exons 4-6, removing a putative catalytic tyrosine encoded in exon 5. Northern blot analysis indicates that the null allele does not express transcripts.
Gonads from homozygous adult males are significantly reduced in weight. Spermatocytes undergo apoptotic death during early prophase. Oocytes from homozygous females progress to the diplotene/dictyate stage in nearly normal numbers, but most die soon after birth. Homozygotes also demonstrate defects in meiocyte homologous chromosomes and are unable to initiate meiotic recombination. Axial elements appear to be normal, but homologous synapsis is impaired.
Heterozygotes are fully fertile, but homozygous males and females are not. Homozygotes are born at predicted Mendelian ratios, indicating their viability is not affected.
A targeting vector containing a neomycin resistance cassette was used to replace exons 4 to 6. The construct was electroporated into 129X1/SvJ-derived embryonic stem (ES) cells (Genome Systems). Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6J for 29 generations by the donating lab.
Allele Name | targeted mutation 1, Maria Jasin |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Spotm1M; Spo11-; Spo11tm1Sky |
Gene Symbol and Name | Spo11, SPO11 meiotic protein covalently bound to DSB |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 2 |
Molecular Note | Exons 4-6 of this gene were replaced with a neomycin resistance cassette via homologous recombination thus deleting the putative catalytic tyrosine in exon 5. Northern blot assay failed to detect gene transcripts in testes of homozygous mutant males. |
When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes are viable but infertile.
When using the B6.129X1-Spo11tm1Mjn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #019117 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Spo11<tm1Mjn> |
Frozen Mouse Embryo | B6.129X1-Spo11<tm1Mjn>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Spo11<tm1Mjn>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Spo11<tm1Mjn>/J | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Spo11<tm1Mjn>/J | $3373.50 |
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