These mice possess loxP sites flanking exons 2 and 3 of the budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae), Bub1, gene and have applications in studies related to mitotic spindle checkpoint and cancer.
Terry Van Dyke, NIH
The serine/threonine kinase encoded by Bub1 is critical to spindle-assembly checkpoint signaling and correct chromosome alignment. These mice possess loxP sites on either side of exons 2 and 3 of the Bub1, budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae), gene. Mice that are homozygous for this allele are viable and fertile.
When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.
|Allele Name||targeted mutation 1, Terry Van Dyke|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Bub1, BUB1, mitotic checkpoint serine/threonine kinase|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A loxP site was inserted into intron 1 and a floxed and frt-flanked neo cassette was inserted into intron 3. Germ line, cre-mediated recombination was used to remove the neo cassette leaving exons 2 and 3 floxed.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129P2(Cg)-Bub1tm1Tvd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #019105 in your Materials and Methods section.