These mice possess loxP sites flanking exons 2 and 3 of the budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae), Bub1, gene and have applications in studies related to mitotic spindle checkpoint and cancer.
Terry Van Dyke, NIH
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Bub1 | BUB1, mitotic checkpoint serine/threonine kinase |
The serine/threonine kinase encoded by Bub1 is critical to spindle-assembly checkpoint signaling and correct chromosome alignment. These mice possess loxP sites on either side of exons 2 and 3 of the Bub1, budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae), gene. Mice that are homozygous for this allele are viable and fertile.
When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.
Allele Name | targeted mutation 1, Terry Van Dyke |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Bub1f |
Gene Symbol and Name | Bub1, BUB1, mitotic checkpoint serine/threonine kinase |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 2 |
Molecular Note | A loxP site was inserted into intron 1 and a floxed and frt-flanked neo cassette was inserted into intron 3. Germ line, cre-mediated recombination was used to remove the neo cassette leaving exons 2 and 3 floxed. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129P2(Cg)-Bub1tm1Tvd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #019105 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Bub1<tm1Tvd> |
Frozen Mouse Embryo | B6.129P2(Cg)-Bub1<tm1Tvd>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2(Cg)-Bub1<tm1Tvd>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2(Cg)-Bub1<tm1Tvd>/J | $3373.50 |
Frozen Mouse Embryo | B6.129P2(Cg)-Bub1<tm1Tvd>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.