B6.129P2(Cg)-Bub1^tm1Tvd/J

Stock number: 019105
Research areas: Research Tools

Description

These mice possess loxP sites flanking exons 2 and 3 of the budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae), Bub1, gene and have applications in studies related to mitotic spindle checkpoint and cancer.

Detailed description

The serine/threonine kinase encoded by Bub1 is critical to spindle-assembly checkpoint signaling and correct chromosome alignment. These mice possess loxP sites on either side of exons 2 and 3 of the Bub1, budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae), gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.

Allele

Symbol: Bub1^tm1Tvd
Name: targeted mutation 1, Terry Van Dyke
MGI accession ID: MGI:3830858
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Bub1^f
Marker symbol: Bub1
Marker name: BUB1, mitotic checkpoint serine/threonine kinase
Marker synonyms: Bub1a; BUB1L; D2Xrf87; hBUB1; MCPH30
Chromosome: 2
Strain of origin: 129P2/OlaHsd
Molecular note: A loxP site was inserted into intron 1 and a floxed and frt-flanked neo cassette was inserted into intron 3. Germ line, cre-mediated recombination was used to remove the neo cassette leaving exons 2 and 3 floxed.