Overview

Also Known As:TRE-KLF4

These transgenic mice express human KLF4, Kruppel-like factor 4, under the direction of the tetracycline operator promoter (tetO). Following DOX induction, bitransgenic mice exhibit hyperkeratosis, atrophy of sebaceous glands, cystic degeneration of hair follicles and hyperplasia. This strain may be useful for studying squamous cell carcinoma (SCC).

Donating Investigator

Michael Ruppert, West Virginia University

Genetic overview

Genetic Background	Generation

Tg(tetO-KLF4)32831Rup

Allele Type
Transgenic (Inducible, Inserted expressed sequence, Humanized sequence)

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Research Applications

Research Tools

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Details

Detailed Description

These transgenic mice express human KLF4, Kruppel-like factor 4, under the direction of the tetracycline operator promoter (tetO). Mice hemizygous for the transgenic insert are viable and fertile. It is not known if homozygotes are viable. When mated to mice carrying K14-rtTA (also known as Tg(KRT14-rtTA)208Jek)), KLF4 expression is directed to basal keratinocytes and regulated with tetracycline or its analog doxycycline (DOX). Following DOX induction, bitransgenic mice exhibit hyperkeratosis, atrophy of sebaceous glands, cystic degeneration of hair follicles and hyperplasia. This strain may be useful for studying squamous cell carcinoma (SCC).

Development

A 4.4 kB transgenic construct containing a tetracycline responsive element (TRE or tetO), the minimal human cytomegalovirus promoter, and the protein coding region of the wildtype human KLF4, Kruppel-like factor 4 was injected into fertilized (C57BL/6 x SJL)F2 mouse eggs. Founder line 32831 was subsequently established. Founders were bred to (C57BL/6 x SJL)F2 mice and then to FVB/NJ for at least 8 generations. Upon arrival at The Jackson Laboratory Repository, the mice were bred with FVB/NJ mice for at least one generation to establish the colony.

Expression Data

Expressed Gene	KLF4, Kruppel like factor 4, human
Site of Expression

Control Suggestions

001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Selected References

Foster KW; Liu Z; Nail CD; Li X; Fitzgerald TJ; Bailey SK; Frost AR; Louro ID; Townes TM; Paterson AJ; Kudlow JE; Lobo-Ruppert SM; Ruppert JM. 2005. Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia. Oncogene 24(9):1491-500PubMed: 15674344 MGI: J:96886

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Genetics

Tg(tetO-KLF4)32831Rup

Allele Symbol: Tg(tetO-KLF4)32831Rup

Allele Name	transgene insertion 32831, J Michael Ruppert
Allele Type	Transgenic (Inducible, Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	TRE-KLF4; TRE-KLF4³²⁸³¹
Gene Symbol and Name	Tg(tetO-KLF4)32831Rup, transgene insertion 32831, J Michael Ruppert
Gene Synonym(s)
Promoter	tetO, tet operator,
Expressed Gene	KLF4, Kruppel like factor 4, human
Strain of Origin	(C57BL/6 x SJL)F2
Chromosome	UN
Molecular Note	The protein-coding region of the human Kruppel-like factor gene KLF4 was ligated to a vector fragment containing a tetracycline-responsive element and the minimal human cytomegalovirus promoter to produce the transgene construct. The 4.4 kb fragment was injected into (C57BL/6 x SJL)F2 ova.
Mutations Made By	Michael Ruppert, West Virginia University

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cancer Research
  - Tetop Tet System

Mammalian Phenotype Terms by Genotype

References

Foster KW; Liu Z; Nail CD; Li X; Fitzgerald TJ; Bailey SK; Frost AR; Louro ID; Townes TM; Paterson AJ; Kudlow JE; Lobo-Ruppert SM; Ruppert JM. 2005. Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia. Oncogene 24(9):1491-500PubMed: 15674344 MGI: J:96886

Additional - Tg(tetO-KLF4)32831Rup related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tg(tetO-KLF4)32831Rup
- Separated PCR:Tg(tetO-KLF4)32831Rup
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as hemizygotes. The donating investigator has not attempted to make the colony homozygous.
- Additional Breeding and Husbandry Support
Citation
When using the TRE-KLF4 mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36730 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:TRE-KLF4

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(tetO-KLF4)32831Rup

Allele Symbol: Tg(tetO-KLF4)32831Rup

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Tg(tetO-KLF4)32831Rup related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information