These transgenic mice express human KLF4, Kruppel-like factor 4, under the direction of the tetracycline operator promoter (tetO). Following DOX induction, bitransgenic mice exhibit hyperkeratosis, atrophy of sebaceous glands, cystic degeneration of hair follicles and hyperplasia. This strain may be useful for studying squamous cell carcinoma (SCC).
Michael Ruppert, West Virginia University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
These transgenic mice express human KLF4, Kruppel-like factor 4, under the direction of the tetracycline operator promoter (tetO). Mice hemizygous for the transgenic insert are viable and fertile. It is not known if homozygotes are viable. When mated to mice carrying K14-rtTA (also known as Tg(KRT14-rtTA)208Jek)), KLF4 expression is directed to basal keratinocytes and regulated with tetracycline or its analog doxycycline (DOX). Following DOX induction, bitransgenic mice exhibit hyperkeratosis, atrophy of sebaceous glands, cystic degeneration of hair follicles and hyperplasia. This strain may be useful for studying squamous cell carcinoma (SCC).
A 4.4 kB transgenic construct containing a tetracycline responsive element (TRE or tetO), the minimal human cytomegalovirus promoter, and the protein coding region of the wildtype human KLF4, Kruppel-like factor 4 was injected into fertilized (C57BL/6 x SJL)F2 mouse eggs. Founder line 32831 was subsequently established. Founders were bred to (C57BL/6 x SJL)F2 mice and then to FVB/NJ for at least 8 generations. Upon arrival at The Jackson Laboratory Repository, the mice were bred with FVB/NJ mice for at least one generation to establish the colony.
Expressed Gene | KLF4, Kruppel like factor 4, human |
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Site of Expression |
Allele Name | transgene insertion 32831, J Michael Ruppert |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | TRE-KLF4; TRE-KLF432831 |
Gene Symbol and Name | Tg(tetO-KLF4)32831Rup, transgene insertion 32831, J Michael Ruppert |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | KLF4, Kruppel like factor 4, human |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | The protein-coding region of the human Kruppel-like factor gene KLF4 was ligated to a vector fragment containing a tetracycline-responsive element and the minimal human cytomegalovirus promoter to produce the transgene construct. The 4.4 kb fragment was injected into (C57BL/6 x SJL)F2 ova. |
Mutations Made By | Michael Ruppert, West Virginia University |
While maintaining a live colony, these mice are bred as hemizygotes. The donating investigator has not attempted to make the colony homozygous.
When using the TRE-KLF4 mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36730 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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