These transgenic mice express human GLI1, GLI1-Kruppel family member GLI1, under the direction of the tetracycline operator promoter (tetO). Following DOX induction, bitransgenic mice exhibit dorsal hair loss and skin lesions characterized by nests of basaloid cells budding from the epidermis. This strain may be useful for studying cutaneous basal cell carcinoma (BCC).
Michael Ruppert, West Virginia University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
These transgenic mice express human GLI1, GLI1-Kruppel family member GLI1, under the direction of the tetracycline operator promoter (tetO). Mice hemizygous for the transgenic insert are viable and fertile. It is not known if homozygotes are viable. When mated to mice carrying K14-rtTA (also known as Tg(KRT14-rtTA)208Jek)), GLI1 expression is directed to skin cells and regulated with tetracycline or its analog doxycycline (DOX). Following DOX induction, bitransgenic mice exhibit dorsal hair loss and skin lesions characterized by nests of basaloid cells budding from the epidermis. This strain may be useful for studying cutaneous basal cell carcinoma (BCC).
A 5.7 kB transgenic construct containing a a tetracycline responsive element (TRE or tetO), the minimal human cytomegalovirus promoter, and an HA-tagged wildtype human GLI1, GLI1-Kruppel family member GLI1, was injected into fertilized (C57BL/6 x SJL)F2 mouse eggs. Founder line 10 was subsequently established. Founders were bred to (C57BL/6 x SJL)F2 mice and then to FVB/NJ for 10 generations. Upon arrival at The Jackson Laboratory Repository, the mice were bred with FVB/NJ mice for at least one generation to establish the colony.
Expressed Gene | GLI1, GLI family zinc finger 1, human |
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Site of Expression |
Allele Name | transgene insertion 10, J Ruppert |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | TRE-GLi1 |
Gene Symbol and Name | Tg(tetO-GLI1)10Rup, transgene insertion 10, J Ruppert |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | GLI1, GLI family zinc finger 1, human |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | The 5.7 kB transgenic construct contains a tetracycline responsive element (TRE or tetO), the minimal human cytomegalovirus promoter, and an HA-tagged wildtype human GLI1, GLI1-Kruppel family member GLI1. |
Mutations Made By | Michael Ruppert, West Virginia University |
While maintaining a live colony, these mice are bred as hemizygotes. The donating investigator has not attempted to make the colony homozygous.
When using the FVB.Cg-Tg(tetO-GLI1)10Rup/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36729 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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