STOCK Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir155)Fjsl}/J

Stock number: 018999
Research areas: Cancer Research, Neurobiology Research, Research Tools

Description

This strain is extinct. Please search the JAX^® Mice Database for strains carrying the Mir155 gene.

Allele

Symbol: Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir155)Fjsl}
Name: targeted mutation 1, Frank J Slack
MGI accession ID: MGI:5431136
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Inducible; Inserted expressed sequence; Transactivator
Synonyms: Gt(ROSA)26Sor^{tm1(tetO-Mir155)Fjsl}; mir-155^LSLtTA
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Marker synonyms: beta geo; Gtrgeo26; Gtrosa26; R26; ROSA26; SETD5-AS1; Thumpd3as1
Chromosome: 6
Strain of origin: (C57BL/6J x SJL/J)F2
Expressed genes: tetO,tet operator,; Mir155,microRNA 155,mouse, laboratory
Site of expression: When activated by Cre recombinase, miR-155 expression was evident in the spleen, thymus, and bone marrow.
Molecular note: A construct was inserted into the locus that contained the following elements (5' to 3'): the tetracycline transactivator (tTA), two poly-adenylation sequences, the tetracycline operator with minimal CMV promoter, a floxed neo cassette with 4 poly-adenylation sequences, the mouse transcript, and a poly-adenylation sequence. The locus promoter drives expression of tTA while the tet operator drives expression of the transcript only after cre mediated removal of the floxed neo-STOP cassette that can be repressed by doxycycline treatment.