B6.A-(rs3659787-rs3689494)/GrsrJ

Stock number: 018993
Strain synonyms: Line M
Research areas: Research Tools

Description

This congenic is part of a panel of nested congenics of Chromosome 11 sequence derived from A/J and bred onto C57BL/6J. They are useful for mapping traits that differ between A/J and C57BL/6J and map to Chromosome 11.

Detailed description

This congenic strain was generated as part of a study funded by The March of Dimes to elucidate the genetic basis of gestation length in mice. In particular, this series of nested congenics was created to look for A/J-derived sequence on Chromosome 11 that could modify the gestation length of C57BL/6J. This Chromosome 11 congenic has a gestation length that is not significantly different from C57BL/6J.

Development

Several C57BL/6J-Chr 11^A/J/NaJ female consomic mice, at approximate generation N13F20, were bred with C57BL/6J males and the F1 and F2 offspring were sibling intercrossed. Generation F3 breeders were selected with the aid of SNP marker genotyping to identify the material to produce a panel of nested intervals that vary by the sequence of A/J Chromosome 11 captured on a C57BL/6J background. Each congenic line was produced by successive generations of marker-assisted breeding to homozygosity for the congenic interval. The congenic interval for this strain, which was line M, includes A/J-derived sequence from Chromosome 11 rs3659787 through rs3689494. In 2013 sperm was cryopreserved from homozygous males at generation N1F10