These VEGFR2e3loxP/e3loxP mice may be useful studying angiogenesis and vascular differentiation.
Thomas N. Sato, Nara Institute of Science and Technology
These VEGFR2e3loxP/e3loxP mutant mice possess loxP sites flanking a frt-flanked PGK-neo cassette and exon 3 of the kinase insert domain protein receptor (Kdr) gene. KDR, also known as VEGFR2 (vascular endothelial growth factor receptor 2), is a type III receptor tyrosine kinase which mediates VEGF-induced regulation of angiogenesis, vascular development, vascular permeability, and embryonic haematopoiesis. Homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
A targeting vector was designed to insert a loxP site upstream of exon 3, and a frt-flanked PGK-neo cassette followed by a second loxP site just downstream of exon 3 of the kinase insert domain protein receptor (Kdr) gene. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to CD1 females. Mice heterozygous for this conditional VEGFR2e3loxP/e3loxP allele were bred with Flp transgenic mice to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 2, Thomas N Sato|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Kdrfl; VEGFR2e3loxP; VEGFR2loxP|
|Gene Symbol and Name||Kdr, kinase insert domain protein receptor|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||A loxP site was inserted upstream of exon 3 and an frt flanked pgk-neo cassette followed by a loxP site were inserted downstream of exon 3 via homologous recombination.|
|Mutations Made By|| |
Thomas Sato, Nara Institute of Science and Technology
When maintaining a live colony, homozygous mice may be bred together.
When using the VEGFR2e3loxP mouse strain in a publication, please cite the originating article(s) and include JAX stock #018977 in your Materials and Methods section.