STOCK Kdr^tm1(cre)Sato/J

Stock number: 018976
Product name: FLK1::Cre
Research areas: Research Tools

Description

These Flk1::Cre mice may be useful for generating conditional mutations for fate mapping blood and vascular endothelial cells.

Detailed description

The Flk1::Cre "knock-in" allele has a Cre recombinase gene inserted into exon 1 of the kinase insert domain protein receptor (Kdr) gene, abolishing gene function and placing Cre expression under the control of the Kdr promoter/enhancer elements. KDR, also known as Flk1 or VEGFR2 (vascular endothelial growth factor receptor 2), is a type III receptor tyrosine kinase which mediates VEGF-induced regulation of angiogenesis, vascular development, vascular permeability, and embryonic haematopoiesis. Heterozygous mice are viable and fertile. The donating investigator reports Cre recombinase activity is observed in all embryonic and adult blood and vascular endothelial cells. When bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the VEGFR2-expressing cells in the offspring.

Development

A targeting vector was designed to insert a nuclear-localized Cre recombinase (NLS-Cre) gene and polyA signal followed by a PGK-Neo cassette into exon 1 of the kinase insert domain protein receptor (Kdr) gene. This construct was electroporated into 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺-derived CJ7 embryonic stem (ES) cells. Chimeric Flk1::Cre mice were bred to CD1 mice to establish the colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation.

Allele

Symbol: Kdr^tm1(cre)Sato
Name: targeted mutation 1, Thomas N Sato
MGI accession ID: MGI:2655253
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Kdr
Marker name: kinase insert domain protein receptor
Chromosome: 5
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase activity is observed in all embryonic and adult blood and vascular endothelial cells.
Molecular note: A knock-in allele was created by replacement of exon 1 with an NLS-cre-PGK-neo cassette via homologous recombination.