These Flk1::Cre mice may be useful for generating conditional mutations for fate mapping blood and vascular endothelial cells.
Thomas N. Sato, Nara Institute of Science and Technology
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing) | Kdr | kinase insert domain protein receptor |
The Flk1::Cre "knock-in" allele has a Cre recombinase gene inserted into exon 1 of the kinase insert domain protein receptor (Kdr) gene, abolishing gene function and placing Cre expression under the control of the Kdr promoter/enhancer elements. KDR, also known as Flk1 or VEGFR2 (vascular endothelial growth factor receptor 2), is a type III receptor tyrosine kinase which mediates VEGF-induced regulation of angiogenesis, vascular development, vascular permeability, and embryonic haematopoiesis. Heterozygous mice are viable and fertile. The donating investigator reports Cre recombinase activity is observed in all embryonic and adult blood and vascular endothelial cells. When bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the VEGFR2-expressing cells in the offspring.
A targeting vector was designed to insert a nuclear-localized Cre recombinase (NLS-Cre) gene and polyA signal followed by a PGK-Neo cassette into exon 1 of the kinase insert domain protein receptor (Kdr) gene. This construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived CJ7 embryonic stem (ES) cells. Chimeric Flk1::Cre mice were bred to CD1 mice to establish the colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | Cre recombinase activity is observed in all embryonic and adult blood and vascular endothelial cells. |
Allele Name | targeted mutation 1, Thomas N Sato |
---|---|
Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Flk1::Cre |
Gene Symbol and Name | Kdr, kinase insert domain protein receptor |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase activity is observed in all embryonic and adult blood and vascular endothelial cells. |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 5 |
Molecular Note | A knock-in allele was created by replacement of exon 1 with an NLS-cre-PGK-neo cassette via homologous recombination. |
Mutations Made By | Thomas Sato, Nara Institute of Science and Technology |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony.
When using the FLK1::Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #018976 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Kdr<tm1(cre)Sato> |
Frozen Mouse Embryo | STOCK Kdr<tm1(cre)Sato>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Kdr<tm1(cre)Sato>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Kdr<tm1(cre)Sato>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Kdr<tm1(cre)Sato>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.