To be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, The Jackson Laboratory Repository chose several Cre recombinase-expressing strains and backcrossed them onto the C57BL/6N genetic background using a marker-assisted, speed-congenic approach.  This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome.  This analysis determined that the colony has at least 99% of the SNP markers as C57BL/6N allele-type.

<p>The parental line, C57BL/6-congenic alphaMhc-Cre transgenic mice, are available and described as Stock No. <a href="https://www.jax.org/strain/011038">011038</a>. It should be noted that the phenotype of these C57BL/6NJ-congenic alphaMhc-Cre transgenic mice (Stock No. 018972) could vary from that of the C57BL/6-congenic parental line from which it was derived. We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.

The cardiac-specific alpha myosin-heavy chain (<i>Myh6</i>) promoter drives expression of Cre recombinase in this alphaMhc-Cre transgenic strain. The promoter induces greater than 90% recombination in cardiac muscle cells.

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B6N.FVB(B6)-Tg(Myh6-cre)2182Mds/J Frozen Embryo