The cardiac-specific alpha myosin-heavy chain (Myh6) promoter drives expression of Cre recombinase in this alphaMhc-Cre transgenic strain. The promoter induces greater than 90% recombination in cardiac muscle cells.
Read More +Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
To be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, The Jackson Laboratory Repository chose several Cre recombinase-expressing strains and backcrossed them onto the C57BL/6N genetic background using a marker-assisted, speed-congenic approach. This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 99% of the SNP markers as C57BL/6N allele-type.
The parental line, C57BL/6-congenic alphaMhc-Cre transgenic mice, are available and described as Stock No. 011038. It should be noted that the phenotype of these C57BL/6NJ-congenic alphaMhc-Cre transgenic mice (Stock No. 018972) could vary from that of the C57BL/6-congenic parental line from which it was derived. We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.
Mice harboring the alphaMhc-Cre transgene on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository in 2009 (as Stock No. 011038). In 2012, some mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain (Stock No. 018972). This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 99% of the SNP markers as C57BL/6N allele-type.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | cre recombinase is expressed in cardiac tissue. |
Allele Name | transgene insertion 2182, Michael D Schneider |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | alphaMhc-Cre; alpha-MHC-Cre+; alphaMyHC-Cre; MCH-cre; MHCalphaCre; MHCcre; MHC-Cre; Tg(Myhca-cre)2182Mds |
Gene Symbol and Name | Tg(Myh6-cre)2182Mds, transgene insertion 2182, Michael D Schneider |
Gene Synonym(s) | |
Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | cre recombinase is expressed in cardiac tissue. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The transgenic construct contained the Myh7 3' untranslated region, the Myhca promoter, Myhca noncoding exons 1 and 2, and the exon 3 splice acceptor site 180 bp upstream and driving the expression of the cre recombinase sequence. The Myhca promoter drives expression in cardiac tissue. |
Mutations Made By | Michael Schneider, Imperial College London |
Mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain. When maintaining the live congenic colony, hemizygous mice may be bred with wildtype (noncarrier) mice from the colony or with C57BL/6NJ inbred mice.
When using the αMyHC-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #018972 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Myh6-cre)2182Mds |
Frozen Mouse Embryo | B6N.FVB(B6)-Tg(Myh6-cre)2182Mds/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.FVB(B6)-Tg(Myh6-cre)2182Mds/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.FVB(B6)-Tg(Myh6-cre)2182Mds/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.FVB(B6)-Tg(Myh6-cre)2182Mds/J Frozen Embryo | $3373.50 |
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