B6N.FVB(B6)-Tg(Myh6-cre)2182Mds/J

Stock number: 018972
Product name: αMyHC-Cre
Research areas: Cardiovascular Research, Research Tools

Description

The cardiac-specific alpha myosin-heavy chain (Myh6) promoter drives expression of Cre recombinase in this alphaMhc-Cre transgenic strain. The promoter induces greater than 90% recombination in cardiac muscle cells.

The alphaMhc-Cre transgenic line 2182 is available on FVB (Stock No. 011037), on C57BL/6 (Stock No. 011038) and backcrossed onto C57BL/6NJ (Stock No. 018972).

Detailed description

To be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, The Jackson Laboratory Repository chose several Cre recombinase-expressing strains and backcrossed them onto the C57BL/6N genetic background using a marker-assisted, speed-congenic approach. This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 99% of the SNP markers as C57BL/6N allele-type.

The parental line, C57BL/6-congenic alphaMhc-Cre transgenic mice, are available and described as Stock No. 011038. It should be noted that the phenotype of these C57BL/6NJ-congenic alphaMhc-Cre transgenic mice (Stock No. 018972) could vary from that of the C57BL/6-congenic parental line from which it was derived. We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.

Development

Mice harboring the alphaMhc-Cre transgene on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository in 2009 (as Stock No. 011038). In 2012, some mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain (Stock No. 018972). This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 99% of the SNP markers as C57BL/6N allele-type.

Allele

Symbol: Tg(Myh6-cre)2182Mds
Name: transgene insertion 2182, Michael D Schneider
MGI accession ID: MGI:2386742
Generation method: Transgenic
Attributes: Recombinase-expressing
Synonyms: alphaMhc-Cre; alpha-MHC-Cre⁺; alphaMyHC-Cre; MCH-cre; MHCalphaCre; MHCcre; MHC-Cre; Tg(Myhca-cre)2182Mds
Marker symbol: Tg(Myh6-cre)2182Mds
Marker name: transgene insertion 2182, Michael D Schneider
Marker synonyms: alphaMhc-Cre; alpha-MHC-Cre⁺; alphaMyHC-Cre; MHCalphaCre; MHCcre; MHC-Cre; Tg(Myhca-cre)2182Mds
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: cre recombinase is expressed in cardiac tissue.
Molecular note: The transgenic construct contained the Myh7 3' untranslated region, the Myhca promoter, Myhca noncoding exons 1 and 2, and the exon 3 splice acceptor site 180 bp upstream and driving the expression of the cre recombinase sequence. The Myhca promoter drives expression in cardiac tissue.