B6N.Cg-Tg(Ins2-cre)25Mgn/J

Stock number: 018960
Product name: Rip-cre
Research areas: Diabetes and Obesity Research, Research Tools

Description

The RIP-Cre transgene has a 668 bp fragment of the rat insulin II promoter, nuclear-localized Cre recombinase, and a 2.1 kbp fragment from the human growth hormone gene. These RIP-Cre transgenic mice are a Cre-lox tool useful for deletion of floxed sequences in pancreatic beta cells.

Detailed description

To be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, The Jackson Laboratory Repository chose several Cre recombinase-expressing strains and backcrossed them onto the C57BL/6N genetic background using a marker-assisted, speed-congenic approach. This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 97% of the SNP markers as C57BL/6N allele-type.

It should be noted that the phenotype of these C57BL/6NJ-congenic RIP-Cre transgenic mice (Stock No. 018960) could vary from that of the parental line originally described on a different genetic background. We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.

Development

Mice harboring the RIP-Cre transgene on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository in 1999 (as Stock No. 003573). In 2012, some mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain (Stock No. 018960). This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 97% of the SNP markers as C57BL/6N allele-type.

Allele

Symbol: Tg(Ins2-cre)25Mgn
Name: transgene insertion 25, Mark A Magnuson
MGI accession ID: MGI:2176227
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Ins2-cre)25Mgn
Marker name: transgene insertion 25, Mark A Magnuson
Chromosome: 7
Strain of origin: (C57BL/6 x DBA)F2
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: pancreatic beta cells
Molecular note: This transgene expresses Cre recombinase with a human growth hormone polyadenylation signal under the control of the rat insulin promoter (Ins2), which is active in pancreatic beta cells and in neurons of the hypothalamus. 9 copies of the transgene are estimated to be present. Transgene insertion occurred on Chr 7, causing a 17400 bp duplication.