The RIP-Cre transgene has a 668 bp fragment of the rat insulin II promoter, nuclear-localized Cre recombinase, and a 2.1 kbp fragment from the human growth hormone gene. These RIP-Cre transgenic mice are a Cre-lox tool useful for deletion of floxed sequences in pancreatic beta cells.
Read More +Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
To be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, The Jackson Laboratory Repository chose several Cre recombinase-expressing strains and backcrossed them onto the C57BL/6N genetic background using a marker-assisted, speed-congenic approach. This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 97% of the SNP markers as C57BL/6N allele-type.
It should be noted that the phenotype of these C57BL/6NJ-congenic RIP-Cre transgenic mice (Stock No. 018960) could vary from that of the parental line originally described on a different genetic background. We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.
Mice harboring the RIP-Cre transgene on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository in 1999 (as Stock No. 003573). In 2012, some mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain (Stock No. 018960). This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 97% of the SNP markers as C57BL/6N allele-type.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | pancreatic beta cells |
Allele Name | transgene insertion 25, Mark A Magnuson |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | [RIP]-Cre; Ins2cre; Ins-Cre; M line; RIP Cre; RIP2Cre; RIP2-cre; RIPCre; Rip-cre; TgN(ins2-Cre)25Mgn |
Gene Symbol and Name | Tg(Ins2-cre)25Mgn, transgene insertion 25, Mark A Magnuson |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | pancreatic beta cells |
Strain of Origin | (C57BL/6 x DBA)F2 |
Chromosome | 7 |
Molecular Note | This transgene expresses Cre recombinase with a human growth hormone polyadenylation signal under the control of the rat insulin promoter (Ins2), which is active in pancreatic beta cells and in neurons of the hypothalamus. 9 copies of the transgene are estimated to be present. Transgene insertion occurred on Chr 7, causing a 17400 bp duplication. |
Mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
When using the Rip-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #018960 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Ins2-cre)25Mgn |
Frozen Mouse Embryo | B6N.Cg-Tg(Ins2-cre)25Mgn/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.Cg-Tg(Ins2-cre)25Mgn/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.Cg-Tg(Ins2-cre)25Mgn/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.Cg-Tg(Ins2-cre)25Mgn/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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