These Krt6-cre/cerulean transgenic mice express cre recombinase and the cerulean variant of green fluorescent protein (GFP) driven by keratin 6 (Krt6) promoter/enhancer elements. They may be useful for generating conditional mutations for studying craniofacial development.
Steve Murray, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
In the laboratory of Dr. Steve Murray at the Jackson Laboratory, a transgene was designed to contain (from 5' to 3') the entire mouse keratin 6 (Krt6) gene, a viral 2A oligopeptide (to mediate ribosomal skipping), cre recombinase, another 2A oligopeptide, the cerulean variant of green fluorescent protein (GFP), and a bovine polyadenylation sequence. This sequence was followed by a frt-flanked neomycin (neo) resistance cassette. This transgene was microinjected into fertilized C57BL/6J oocytes and founder male 5 was bred to C57BL/6J females (Stock No. 000664). Upon arrival in The Jackson Laboratory Repository, mice were bred to C57BL/6J inbred mice for at least one generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Keratin 6 (Krt6) promoter/enhancer elements drive the expression of cre recombinase and the cerulean variant of green fluorescent protein in epidermal cells from early stages of epidermis formation through adulthood. |
Allele Name | transgene insertion 5, Genetic Resource Science Repository |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Krt6,-cre,-Cerulean)5Grsr, transgene insertion 5, Genetic Resource Science Repository |
Gene Synonym(s) | |
Promoter | Krt6, keratin 6, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Keratin 6 (Krt6) promoter/enhancer elements drive the expression of cre recombinase and the cerulean variant of green fluorescent protein in epidermal cells from early stages of epidermis formation through adulthood. |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | a transgene was designed to contain (from 5' to 3') the entire mouse keratin 6 (Krt6) gene, a viral 2A oligopeptide (to mediate ribosomal skipping), cre recombinase, another 2A oligopeptide, the cerulean variant of green fluorescent protein (GFP), and a bovine polyadenylation sequence. This sequence was followed by a frt-flanked neomycin (neo) resistance cassette. This transgene was microinjected into fertilized C57BL/6J oocytes and founder male 5 was bred to establish line 5. |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the Krt6-cre/cerulean mouse strain in a publication, please cite the originating article(s) and include JAX stock #018899 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizyous or Non Carrir for Tg(Krt6,-cre,-Cerulean)5Grsr |
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