C57BL/6J-Tg(Krt6,-cre,-Cerulean)5Grsr/Grsr

Stock number: 018899
Product name: Krt6-cre/cerulean
Research areas: Developmental Biology Research, Research Tools

Description

These Krt6-cre/cerulean transgenic mice express cre recombinase and the cerulean variant of green fluorescent protein (GFP) driven by keratin 6 (Krt6) promoter/enhancer elements. They may be useful for generating conditional mutations for studying craniofacial development.

Development

In the laboratory of Dr. Steve Murray at the Jackson Laboratory, a transgene was designed to contain (from 5' to 3') the entire mouse keratin 6 (Krt6) gene, a viral 2A oligopeptide (to mediate ribosomal skipping), cre recombinase, another 2A oligopeptide, the cerulean variant of green fluorescent protein (GFP), and a bovine polyadenylation sequence. This sequence was followed by a frt-flanked neomycin (neo) resistance cassette. This transgene was microinjected into fertilized C57BL/6J oocytes and founder male 5 was bred to C57BL/6J females (Stock No. 000664). Upon arrival in The Jackson Laboratory Repository, mice were bred to C57BL/6J inbred mice for at least one generation to establish the colony.

Allele

Symbol: Tg(Krt6,-cre,-Cerulean)5Grsr
Name: transgene insertion 5, Genetic Resource Science Repository
MGI accession ID: MGI:5485017
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Krt6,-cre,-Cerulean)5Grsr
Marker name: transgene insertion 5, Genetic Resource Science Repository
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: GFP,Green Fluorescent Protein,; cre,cre recombinase,bacteriophage P1
Site of expression: Keratin 6 (Krt6) promoter/enhancer elements drive the expression of cre recombinase and the cerulean variant of green fluorescent protein in epidermal cells from early stages of epidermis formation through adulthood.
Molecular note: a transgene was designed to contain (from 5' to 3') the entire mouse keratin 6 (Krt6) gene, a viral 2A oligopeptide (to mediate ribosomal skipping), cre recombinase, another 2A oligopeptide, the cerulean variant of green fluorescent protein (GFP), and a bovine polyadenylation sequence. This sequence was followed by a frt-flanked neomycin (neo) resistance cassette. This transgene was microinjected into fertilized C57BL/6J oocytes and founder male 5 was bred to establish line 5.