STOCK Dchs1^tm1.1Irv/J

Stock number: 018851
Research areas: Research Tools

Description

These Dchs1 mice possess loxP sites flanking exon 2 of the dachsous 1 (Drosophila) gene. They are useful for generating conditional mutations for studies related to the development of multiple organs/tissues including the kidney, heart, lung and skeleton.

Detailed description

These mice possess loxP sites on either side of exon 2 of the Dchs1 gene. The flanked region involves the first six cadherin domains as well as the initiator methionine and signal peptide.When these mice are bred to mice that express Cre recombinase, the resulting offspring will have the flanked sequence deleted in cre-expressing tissues.

Widespread knockout of Dchs1 (e.g. through crosses with the Tg(Sox2-cre)1Amc strain (Stock No. 004783)) causes defects in multiple internal organs, including kidney, heart, lung and skeleton. Cochleas of homozygous knockouts are shorter than those of wildtype mice. The kidneys are small and cystic. At birth, the lungs are smaller and the intestinal length is reduced. The skeleton exhibits abnormal ossification patterns in the sternum. Although born at predicted Mendelian ratios, homozygous mutants exhibit a variable neonatal lethality, sometimes surviving for two weeks. Homozygous knockout pups are similar in size to their wildtype siblings, but they often have a bent spine and curly tails. They show some signs of postnatal development, such as hair formation, but fail to grow.

Development

Exon 2 was flanked by loxP sites and preceded by a 5' FRT-flanked PGK-neomycin cassette. The mutation was introduced to AB2.2 129S7/SvEvBrd-Hprt1⁺-derived embryonic stem (ES) cells. Chimeric mice were crossed to C57BL/6J to produce conditional targeting mice. The PGK-neomycin marker was deleted by crossing to 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym/J (see Stock No. 003946). This strain was maintained on a mixed 129, C57BL/6 and FVB genetic background by the donating laboratory.

Allele

Symbol: Dchs1^tm1.1Irv
Name: targeted mutation 1.1, Kenneth Irvine
MGI accession ID: MGI:5428667
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Dchs1
Marker name: dachsous cadherin related 1
Chromosome: 7
Strain of origin: 129S7/SvEvBrd-Hprt^b-m2
Molecular note: Exon 2 was flanked by loxP sites and preceded by a 5' FRT-flanked PGK-neomycin cassette. Flp-mediated recombination removed the neo cassette and left exon 2 floxed.