These Dchs1 mice possess loxP sites flanking exon 2 of the dachsous 1 (Drosophila) gene. They are useful for generating conditional mutations for studies related to the development of multiple organs/tissues including the kidney, heart, lung and skeleton.
Kenneth Irvine, Waksman Institute, Rutgers University
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Dchs1 | dachsous cadherin related 1 |
These mice possess loxP sites on either side of exon 2 of the Dchs1 gene. The flanked region involves the first six cadherin domains as well as the initiator methionine and signal peptide.When these mice are bred to mice that express Cre recombinase, the resulting offspring will have the flanked sequence deleted in cre-expressing tissues.
Widespread knockout of Dchs1 (e.g. through crosses with the Tg(Sox2-cre)1Amc strain (Stock No. 004783)) causes defects in multiple internal organs, including kidney, heart, lung and skeleton. Cochleas of homozygous knockouts are shorter than those of wildtype mice. The kidneys are small and cystic. At birth, the lungs are smaller and the intestinal length is reduced. The skeleton exhibits abnormal ossification patterns in the sternum. Although born at predicted Mendelian ratios, homozygous mutants exhibit a variable neonatal lethality, sometimes surviving for two weeks. Homozygous knockout pups are similar in size to their wildtype siblings, but they often have a bent spine and curly tails. They show some signs of postnatal development, such as hair formation, but fail to grow.
Exon 2 was flanked by loxP sites and preceded by a 5' FRT-flanked PGK-neomycin cassette. The mutation was introduced to AB2.2 129S7/SvEvBrd-Hprt1+-derived embryonic stem (ES) cells. Chimeric mice were crossed to C57BL/6J to produce conditional targeting mice. The PGK-neomycin marker was deleted by crossing to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (see Stock No. 003946). This strain was maintained on a mixed 129, C57BL/6 and FVB genetic background by the donating laboratory.
Allele Name | targeted mutation 1.1, Kenneth Irvine |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Dchs1, dachsous cadherin related 1 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 7 |
Molecular Note | Exon 2 was flanked by loxP sites and preceded by a 5' FRT-flanked PGK-neomycin cassette. Flp-mediated recombination removed the neo cassette and left exon 2 floxed. |
Mutations Made By | Kenneth Irvine, Waksman Institute, Rutgers University |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the STOCK Dchs1tm1.1Irv/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018851 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Dchs1<tm1.1Irv> |
Frozen Mouse Embryo | STOCK Dchs1<tm1.1Irv>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Dchs1<tm1.1Irv>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Dchs1<tm1.1Irv>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Dchs1<tm1.1Irv>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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