Homozygous Sfrp2 (secreted frizzled-related protein 2) mutant mice show a very mild skeletal phenotype affecting distal portions of the limbs (autopods) with shortening of metacarpals/metatarsals and all phalangeal elements. In addition there is soft-tissue syndactyly in the hind limbs between the 3rd and 4th digits (monolateral or bilateral).
Brendan Lee, Baylor College of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Sfrp2 | secreted frizzled-related protein 2 |
These Sfrp2 (secreted frizzled-related protein 2) mutant mice carry a knockout allele that is useful in delineating the role of the gene in embryonic and skeletal development.
Homozygous animals are undistinguishable from their wild-type littermates at birth and exhibit normal patterning of mesenchymal cell condensations (skeletal progenitors) with normal joint formation. They show, however, mild mesomelic (mid-segment) shortening of limbs and brachydactyly of metacarpals/metatarsals due to decreased chondrocyte proliferation. They also exhibit delayed differentiation in distal limb skeletal elements, and hindlimb soft-tissue syndactyly between the 3rd and 4th digits (monolateral or bilateral) due to a lack of interdigital mesenchyme regression.
Homozygotes are viable and fertile and have a normal lifespan. Northern blot of total RNA from whole embryos at E15.5 demonstrates the absence of transcript expression from the mutant allele.
Exons 1 and 2 were replaced by a loxP-flanked TK-neomycin cassette in AB2.2 129S7/SvEvBrd-Hprt1+-derived embryonic stem (ES) cells. Recombinant ES cell clones were further transfected with Cre-recombinase and the floxed cassette was removed. Only exon 3 of the gene remains. The donating investigator reported that chimeric males were crossed with a 129 inbred female to initiate the line. Upon arrival, mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Allele Name | targeted mutation 1.1, Brendan Lee |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Sfrp2, secreted frizzled-related protein 2 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 3 |
Molecular Note | Exons 1 and 2 were replaced by a loxP-flanked TK-neomycin cassette. Cre-mediated recombination removed the neo cassette. Northern blot analysis confirmed the absence of transcript expression. |
Mutations Made By | Brendan Lee, Baylor College of Medicine |
Heterozygotes or homozygotes may be bred.
When using the B6;129-Sfrp2tm1.1Brle/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018832 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Sfrp2<tm1.1Brle> |
Frozen Mouse Embryo | B6;129-Sfrp2<tm1.1Brle>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Sfrp2<tm1.1Brle>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Sfrp2<tm1.1Brle>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Sfrp2<tm1.1Brle>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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