B6;129S7-Crtap^tm1Brle/J

Stock number: 018831
Research areas: Developmental Biology Research, Internal/Organ Research

Description

Homozygous Crtap (cartilage associated protein) knockout mice develop osteochondrodysplasia characterized by severe osteoporosis and decreased osteoid production. This strain may be useful as a model of osteogenesis imperfecta.

Development

A fragment containing the coding sequence of exon 1, intron 1, exon 2, intron 2 and part of exon 3 was replaced with a neomycin cassette using AB2.2 129S7/SvEvBrd-Hprt1⁺-derived embryonic stem (ES) cells. This strain was maintained on a mixed C57BL/6-129 genetic background by the donating laboratory.

Allele

Symbol: Crtap^tm1Brle
Name: targeted mutation 1, Brendan Lee
MGI accession ID: MGI:3693334
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Crtap
Marker name: cartilage associated protein
Chromosome: 9
Strain of origin: 129S7/SvEvBrd-Hprt1^b-m2
Molecular note: A neomycin resistance cassette replaced part of exon 1 and all of exon 2. Absence of transcript was confirmed by Northern blot and RT-PCR analyses.