Mice hemizygous for the BAC LRRK2 (G2019S) transgene express a mutant form of human leucine-rich repeat kinase 2 (LRRK2*G2019S) associated with autosomal dominant, late-onset Parkinson's disease directed by the human LRRK2 promoter/enhancer regions on the BAC transgene. These mice represent an in vivo model for studying the dominant toxic effects of mutant LRRK2*G2019S expression.
Kuldip Dave, The Michael J. Fox Foundation
Genetic Background | Generation |
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N12pN3F7
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
Mice hemizygous for the BAC LRRK2 (G2019S) transgene are viable and fertile, with expression of a mutant form of human leucine-rich repeat kinase 2 (LRRK2*G2019S) associated with autosomal dominant, late-onset Parkinson's disease directed by the human LRRK2 promoter/enhancer regions on the BAC transgene. These mice represent an in vivo model for studying the dominant toxic effects of mutant LRRK2*G2019S expression.
Distribution of this strain was supported by the Michael J. Fox Foundation for Parkinson's Research.
A bacterial artificial chromosome (BAC) containing the entire human LRRK2 (leucine-rich repeat kinase 2) gene was modified by targeted mutation of the LRRK2 locus to harbor the LRRK2 G2019S mutation associated with autosomal dominant, late-onset Parkinson's disease originally identified in Spanish families originating from the Basque region. This modified 188 kb BAC was microinjected into fertilized C57BL/6J zygotes. Founder BAC LRRK2*G2019S mice were established and then subsequently maintained by breeding transgenic mice with C57BL/6J (Stock No. 000664) to establish the colony. The transgene integrated on Chr 2.
Expressed Gene | LRRK2, leucine rich repeat kinase 2, human |
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Site of Expression |
Allele Name | transgene insertion 2A, The Michael J Fox Foundation |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | Tg(LRRK2*G2019S)2AGrsr |
Gene Symbol and Name | Tg(LRRK2*G2019S)2AMjff, transgene insertion 2A, The Michael J Fox Foundation |
Gene Synonym(s) | |
Promoter | LRRK2, leucine rich repeat kinase 2, human |
Expressed Gene | LRRK2, leucine rich repeat kinase 2, human |
Strain of Origin | C57BL/6J |
Chromosome | 2 |
Molecular Note | A 188 kb bacterial artificial chromosome (BAC) containing the entire human LRRK2 (leucine-rich repeat kinase 2) gene was modified by targeted mutation of the LRRK2 locus to harbor the LRRK2 G2019S mutation associated with autosomal dominant, late-onset Parkinson's disease originally identified in Spanish families originating from the Basque region. |
When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to C57LB/6J inbred mice (Stock No. 000664).
When using the C57BL/6J-Tg(LRRK2*G2019S)2AMjff/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018785 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(LRRK2*G2019S)2AMjff/ |
Frozen Mouse Embryo | C57BL/6J-Tg(LRRK2*G2019S)2AMjff/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(LRRK2*G2019S)2AMjff/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(LRRK2*G2019S)2AMjff/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Tg(LRRK2*G2019S)2AMjff/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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