C57BL/6J-Tg(LRRK2*G2019S)2AMjff/J

Stock number: 018785
Research areas: Neurobiology Research, Research Tools

Description

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Mice hemizygous for the BAC LRRK2 (G2019S) transgene express a mutant form of human leucine-rich repeat kinase 2 (LRRK2*G2019S) associated with autosomal dominant, late-onset Parkinson's disease directed by the human LRRK2 promoter/enhancer regions on the BAC transgene. These mice represent an in vivo model for studying the dominant toxic effects of mutant LRRK2*G2019S expression.

Detailed description

Mice hemizygous for the BAC LRRK2 (G2019S) transgene are viable and fertile, with expression of a mutant form of human leucine-rich repeat kinase 2 (LRRK2*G2019S) associated with autosomal dominant, late-onset Parkinson's disease directed by the human LRRK2 promoter/enhancer regions on the BAC transgene. These mice represent an in vivo model for studying the dominant toxic effects of mutant LRRK2*G2019S expression.

Distribution of this strain was supported by the Michael J. Fox Foundation for Parkinson's Research.

Development

A bacterial artificial chromosome (BAC) containing the entire human LRRK2 (leucine-rich repeat kinase 2) gene was modified by targeted mutation of the LRRK2 locus to harbor the LRRK2 G2019S mutation associated with autosomal dominant, late-onset Parkinson's disease originally identified in Spanish families originating from the Basque region. This modified 188 kb BAC was microinjected into fertilized C57BL/6J zygotes. Founder BAC LRRK2*G2019S mice were established and then subsequently maintained by breeding transgenic mice with C57BL/6J (Stock No. 000664) to establish the colony. The transgene integrated on Chr 2 (Table S1, Goodwin, 2019, PMID: 30659012).

*Please note that the mapping data in Table 1 of the Goodwin, 2019 paper, placing the transgene on mouse Chr 1, is incorrect (August, 2024)

Allele

Symbol: Tg(LRRK2*G2019S)2AMjff
Name: transgene insertion 2A, The Michael J Fox Foundation
MGI accession ID: MGI:5502368
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Synonyms: Tg(LRRK2*G2019S)2AGrsr
Marker symbol: Tg(LRRK2*G2019S)2AMjff
Marker name: transgene insertion 2A, The Michael J Fox Foundation
Marker synonyms: Tg(LRRK2*G2019S)2AGrsr
Chromosome: 2
Strain of origin: C57BL/6J
Expressed genes: LRRK2,leucine rich repeat kinase 2,human
Molecular note: A 188 kb bacterial artificial chromosome (BAC) containing the entire human LRRK2 (leucine-rich repeat kinase 2) gene was modified by targeted mutation of the LRRK2 locus to harbor the LRRK2 G2019S mutation associated with autosomal dominant, late-onset Parkinson's disease originally identified in Spanish families originating from the Basque region.