The transgenic construct contains the BAC clone RP23-472D11, which includes the wild-type Sfxn1 gene. When combined with mice homozygous for Sfxn1m1Mdf, mice are viable and fertile.
Mark D Fleming, Children's Hospital Boston
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
The transgenic construct contains the BAC clone RP23-472D11, which includes the wild-type Sfxn1 gene. When combined with mice homozygous for Sfxn1m1Mdf, mice are viable and fertile.
The transgenic construct contains the BAC clone RP23-472D11, which includes the wild-type Sfxn1 gene. The transgene was electroporated into 129S4/SvJae embryonic stem cells. Mice from founder line 7 were mated to 129S6.Cg-Sfxn1m1Mdf mice. The donating investigator maintained the strain as a cross to 129S6.Cg-Sfxn1m1Mdf at every generation. The transgene insertion site is unknown. Please Note: The MMRRC at The Jackson Laboratory is importing this strain without the Sfxn1 mutation.
Expressed Gene | SFXN1, sideroflexin 1, human |
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Site of Expression |
Allele Name | transgene insertion 7, Mark D Fleming |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | Tg(472D11) |
Gene Symbol and Name | Tg(RP23-472D11)7Mdf, transgene insertion 7, Mark D Fleming |
Gene Synonym(s) | |
Promoter | SFXN1, sideroflexin 1, human |
Expressed Gene | SFXN1, sideroflexin 1, human |
Strain of Origin | 129S4/SvJae |
Chromosome | UN |
Molecular Note | The transgene contains human bac BAC clone RP23-472D11. Line 7 was generated. |
While maintaining a live colony, these mice are bred as hemizygous for the transgene. The donating investigator has not attempted to make the transgene homozygous.
When using the Tg(472D11) mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36691 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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